CD24 is a marker of exosomes secreted into urine and amniotic fluid

Exosomes are small membrane vesicles that are secreted from a variety of cell types into various body fluids including the blood and urine. These vesicles are thought to play a role in cell–cell interactions. CD24 is a small but extensively glycosylated protein linked to the cell surface by means of...

Full description

Saved in:
Bibliographic Details
Published in:Kidney international 2007-11, Vol.72 (9), p.1095-1102
Main Authors: Keller, S., Rupp, C., Stoeck, A., Runz, S., Fogel, M., Lugert, S., Hager, H.-D., Abdel-Bakky, M.S., Gutwein, P., Altevogt, P.
Format: Article
Language:English
Subjects:
Adult
Aged
amniotic fluid
Amniotic Fluid - metabolism
Animals
Animals, Newborn - urine
Biomarkers - metabolism
Biomarkers - urine
CD24
CD24 Antigen - genetics
CD24 Antigen - metabolism
CD24 Antigen - urine
exosomes
Female
Humans
Infant, Newborn
Kidney Tubules, Proximal - cytology
Kidney Tubules, Proximal - embryology
Kidney Tubules, Proximal - metabolism
Male
Maternal-Fetal Exchange - physiology
Mice
Mice, Inbred C57BL
Mice, Knockout
Middle Aged
podocytes
Podocytes - cytology
Podocytes - metabolism
Pregnancy
Secretory Vesicles - metabolism
urine
Citations: Items that this one cites
Items that cite this one
Online Access:Request full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Exosomes are small membrane vesicles that are secreted from a variety of cell types into various body fluids including the blood and urine. These vesicles are thought to play a role in cell–cell interactions. CD24 is a small but extensively glycosylated protein linked to the cell surface by means of a glycosyl-phosphatidylinositol anchor. In this study we found that CD24 is present in membrane vesicles characterized as exosomes that were isolated from the urine of normal individuals. CD24 was expressed by both tubule cells and podocytes and treatment of the latter with a cholesterol-extracting agent, but not with a calcium ionophore, caused the release of CD24-containing exosomes. Using CD24 as a marker, we found exosomes in the urine of newborn infants and in the amniotic fluid of pregnant women with similar findings made in mice. Interestingly, studies with CD24 knockout mice showed that the exosomes are released from the fetus but not from the mother; however, exosome release was similar from both the knockout and the wild-type mice. This indicates that CD24 is not essential for exosome formation or release but may be a convenient exosome marker. Our studies suggest that exosomal secretion from the embryonic kidney could play a biological role at the fetal–maternal interphase.
ISSN:0085-2538
1523-1755
DOI:10.1038/sj.ki.5002486