Rapid Species Determination of Nocardia Keratitis Using Pyrosequencing Technology

Purpose To describe a new technique, pyrosequencing, which allows for the rapid identification of Mycobacterium and Nocardia species. Design Interventional case report. Methods The medical records of a patient presenting with an infectious keratitis were reviewed. Results A case of Nocardia abscessu...

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Bibliographic Details
Published in:American journal of ophthalmology 2007, Vol.143 (1), p.182-183
Main Authors: Galor, Anat, MD, Hall, Gerri S., PhD, Procop, Gary W., MD, Tuohy, Marion, MT, Millstein, Michael E., MD, Jeng, Bennie H., MD
Format: Article
Language:English
Subjects:
Adult
Anti-Infective Agents - therapeutic use
Antibiotics
Bacterial Typing Techniques
Base Sequence
Biological and medical sciences
Deoxyribonucleic acid
Diseases of cornea, anterior segment and sclera
DNA
DNA, Bacterial - analysis
Eye Infections, Bacterial - diagnosis
Eye Infections, Bacterial - drug therapy
Eye Infections, Bacterial - microbiology
Humans
Keratitis - diagnosis
Keratitis - drug therapy
Keratitis - microbiology
Male
Medical research
Medical sciences
Miscellaneous
Molecular Sequence Data
Nocardia - genetics
Nocardia - isolation & purification
Nocardia Infections - diagnosis
Nocardia Infections - drug therapy
Nocardia Infections - microbiology
Ophthalmic Solutions - therapeutic use
Ophthalmology
Sequence Analysis, DNA - methods
Trimethoprim, Sulfamethoxazole Drug Combination - therapeutic use
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Summary:Purpose To describe a new technique, pyrosequencing, which allows for the rapid identification of Mycobacterium and Nocardia species. Design Interventional case report. Methods The medical records of a patient presenting with an infectious keratitis were reviewed. Results A case of Nocardia abscessus/arthrititis/asiatica keratitis was diagnosed in a young individual with the aid of pyrosequencing technology. Based on presumed antibiotic sensitivities, therapy with topical trimethoprim-sulfamethoxazole eyedrops was initiated, and the infection was cleared rapidly with minimal residual scarring. Conclusions Pyrosequencing may be a useful tool in aiding the rapid diagnosis and treatment of ocular infections caused by slow-growing pathogens.
ISSN:0002-9394
1879-1891
DOI:10.1016/j.ajo.2006.07.036