The effects of GnRH analogue (buserelin) or hCG (Chorulon) on Day 12 of pregnancy on ovarian function, plasma hormone concentrations, conceptus growth and placentation in ewes and ewe lambs

The objectives of this study were to determine the effect of GnRH analogue (buserelin) or human chorionic gonadotrophin (hCG, Chorulon) treatment on Day 12 of pregnancy on ovarian function, plasma hormone concentrations, conceptus growth and placentation in ewes and ewe lambs. After oestrus synchron...

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Bibliographic Details
Published in:Animal reproduction science 2007-12, Vol.102 (3), p.247-257
Main Authors: Khan, T.H., Beck, N.F.G., Khalid, M.
Format: Article
Language:English
Subjects:
Animals
Buserelin - pharmacology
Chorionic Gonadotropin - pharmacology
Conceptus
Embryonic Development - drug effects
Estradiol - blood
Ewe lambs
Ewes
Female
Follicle Stimulating Hormone - secretion
Gestational Age
GnRH
hCG
Hormones - blood
Luteinizing Hormone - secretion
Ovarian function
Ovary - drug effects
Ovary - physiology
Ovulation
Placenta - anatomy & histology
Placentation - drug effects
Pregnancy
Progesterone - blood
Sheep - physiology
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Summary:The objectives of this study were to determine the effect of GnRH analogue (buserelin) or human chorionic gonadotrophin (hCG, Chorulon) treatment on Day 12 of pregnancy on ovarian function, plasma hormone concentrations, conceptus growth and placentation in ewes and ewe lambs. After oestrus synchronization with progestagen sponges and eCG, all the animals were mated with fertile rams. Both ewes and ewe lambs (20 per treatment group) were given either normal saline or 4 μg GnRH or 200 IU hCG on Day 12 post-mating. Pre- and post-treatment plasma hormone concentrations were determined in seven pregnant animals per treatment group in samples collected 1 h before and 0, 2, 4, 6, 8, 24, 48 and 72 h after treatment. Overall mean progesterone concentrations were higher ( P < 0.001) in ewes as compared with ewe lambs in saline-treated controls. GnRH or hCG treatment increased ( P < 0.001) mean plasma progesterone concentrations in both age groups, however, post-treatment concentrations were significantly ( P < 0.05) higher in ewes than in ewe lambs. Oestradiol concentrations were similar in the two control groups. In ewes, but not in ewe lambs, both GnRH and hCG treatments significantly ( P < 0.05) increased the mean oestradiol concentrations above pre-treatment levels. Moreover, post-treatment oestradiol concentrations in GnRH- and hCG-treated animals were significantly ( P < 0.05) higher than those in the saline-treated controls. LH release in response to GnRH treatment was greater ( P < 0.05) in ewes than in ewe lambs, whereas FSH release in ewes was less ( P < 0.05) than that of ewe lambs. The effects of GnRH or hCG on conceptus growth and placentation was determined at slaughter on Day 25. In ewes, GnRH treatment increased ( P < 0.05) luteal weight, amniotic sac width and length, and crown-rump length compared with controls, but had no effect on these parameters in ewe lambs. In ewes, hCG treatment also enhanced ( P < 0.05) luteal weight, amniotic sac width and length, crown-rump length, embryo weight and number of placentomes as compared with controls. In ewe lambs, there was no difference ( P < 0.05) between hCG and control groups in luteal weight, embryo weight and amniotic sac width but crown-rump length, amniotic sac length and the number of placentomes forming the placenta were greater ( P < 0.05). In conclusion, GnRH or hCG treatment on Day 12 of pregnancy can increase ovarian function, conceptus growth and placental attachment in ewes. However, these t
ISSN:0378-4320
1873-2232
DOI:10.1016/j.anireprosci.2006.11.007