A double-blind, randomized quantitative comparison of calcitriol ointment and calcipotriol ointment on epidermal cell populations, proliferation and differentiation

Summary Background  Calcitriol and calcipotriol are widely used in the topical treatment of psoriasis. However, studies comparing both treatment modalities are scarce. Especially, there are almost no studies comparing the effects on epidermal cell populations in a quantitative manner. Objectives  Th...

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Bibliographic Details
Published in:British journal of dermatology (1951) 2007-01, Vol.156 (1), p.130-137
Main Authors: Körver, J.E.M., Vissers, W.H.P.M., Van Rens, D.W.A., Pasch, M.C., Van Erp, P.E.J., Boezeman, J.B.M., Van De Kerkhof, P.C.M.
Format: Article
Language:English
Subjects:
Administration, Topical
Biological and medical sciences
calcipotriol
calcitriol
Calcitriol - analogs & derivatives
Calcitriol - therapeutic use
Calcium Channel Agonists - therapeutic use
Cell Differentiation - drug effects
Cell Proliferation - drug effects
Dermatologic Agents - therapeutic use
Dermatology
Double-Blind Method
Epidermal Growth Factor - drug effects
Humans
Immunohistochemistry
immunohistology
keratin 15
Keratins - metabolism
Medical sciences
Ointments
psoriasis
Psoriasis - drug therapy
Psoriasis - pathology
Psoriasis. Parapsoriasis. Lichen
transit-amplifying cells
Treatment Outcome
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Summary:Summary Background  Calcitriol and calcipotriol are widely used in the topical treatment of psoriasis. However, studies comparing both treatment modalities are scarce. Especially, there are almost no studies comparing the effects on epidermal cell populations in a quantitative manner. Objectives  The aim of this study was to quantitatively compare the effects of topical calcitriol and topical calcipotriol on clinical scores and epidermal subpopulations. Patients and methods  From five patients with stable plaque psoriasis, skin biopsies were taken from two symmetrical regions on the trunk or extremities before and after treatment with either calcitriol or calcipotriol. Frozen sections were labelled immunofluorescently using direct immunofluorescence for β‐1 integrin and the Zenon labelling technique for keratin (K) 6, K10 and K15. The digital photographs of the stained sections were quantitatively analysed and the results of both treatments were compared. Results  The clinical SUM‐score improved significantly for both the calcitriol‐ and the calcipotriol‐treated lesions. In the calcipotriol‐treated group the expression of K10 and K15 increased and the expression of K6 decreased significantly. No changes were seen for the marker β‐1 integrin. In the calcitriol‐treated group none of the markers changed significantly. A tendency towards significance was seen for the changes in the expression of K6 and K15 in favour of calcipotriol. Conclusions  Both calcitriol and calcipotriol gave a significant improvement in clinical scores. However, treatment with calcipotriol resulted in a normalization of K6, K10 and K15, whereas treatment with calcitriol did not. Comparison of both treatments showed a tendency towards significance for the above‐mentioned markers for calcipotriol only.
ISSN:0007-0963
1365-2133
DOI:10.1111/j.1365-2133.2006.07561.x