Noise and kinetics of LuxR positive feedback loops

We have previously reported the design and construction of positive feedback loops (PFLs) based on the LuxI–LuxR quorum-sensing system that can be used as modular transcriptional regulatory units for the construction of complex artificial genetic circuits. Here, we characterize these PFLs using sing...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2007-11, Vol.363 (3), p.667-673
Main Authors: Sayut, Daniel J., Kambam, Pavan Kumar Reddy, Sun, Lianhong
Format: Article
Language:English
Subjects:
Escherichia coli - genetics
Feedback, Physiological
Gene Expression Regulation, Bacterial
Green Fluorescent Proteins - genetics
Green Fluorescent Proteins - metabolism
Kinetics
LuxR
Mutation
Positive feedback
Quorum sensing
Quorum Sensing - genetics
Quorum Sensing - physiology
Recombinant Fusion Proteins - genetics
Recombinant Fusion Proteins - metabolism
Repressor Proteins - genetics
Repressor Proteins - metabolism
Repressor Proteins - physiology
Signal Transduction - genetics
Signal Transduction - physiology
Spectrometry, Fluorescence
Synthetic biology
Time Factors
Trans-Activators - genetics
Trans-Activators - metabolism
Trans-Activators - physiology
Transfection
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Summary:We have previously reported the design and construction of positive feedback loops (PFLs) based on the LuxI–LuxR quorum-sensing system that can be used as modular transcriptional regulatory units for the construction of complex artificial genetic circuits. Here, we characterize these PFLs using single-cell and dynamic induction studies to fully understand their behavior and facilitate their incorporation into novel networks. The LuxR PFLs had graded responses to the OHHL signal molecule with inductions developing over time, causing a lag in response compared to a non-feedback control. The properties of the PFLs could be altered using LuxR mutants with altered sensitivities without changing the inherent properties of the systems. Because of their high sensitivity and ability to establish intercellular signaling, the LuxR PFLs described in this work could be used as well-defined modules for the construction of artificial genetic circuits.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2007.09.057