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Spindle Checkpoint Protein hMad2 and Histone H3 Phosphoserine 10 Mitosis Marker in Pediatric Solid Tumors
Pediatric solid tumors of different histologic types are often treated with drugs (such as vincristine) that directly or indirectly target the mitotic spindle and the spindle checkpoint, leading to mitotic arrest, mitotic catastrophe and apoptosis. hMad2 (mitosis arrest deficient 2) expression in ne...
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Published in: | Anticancer research 2006-11, Vol.26 (6C), p.4687-4694 |
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Main Author: | |
Format: | Article |
Language: | English |
Subjects: | |
Online Access: | Get full text |
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Summary: | Pediatric solid tumors of different histologic types are often treated with drugs (such as vincristine) that directly or indirectly
target the mitotic spindle and the spindle checkpoint, leading to mitotic arrest, mitotic catastrophe and apoptosis. hMad2
(mitosis arrest deficient 2) expression in neuroblastoma has suggested utility in predicting prognosis. The present study
was undertaken to determine hMad2 expression patterns in pediatric solid tumors, and its relationship to overall treatment
response and short-term survival (2-years). The expression of hMad2 and histone H3-serine 10 phosphoprotein (mitosis marker)
were examined using immunohistochemical staining methods in 26 pediatric solid tumors (six neuroblastomas, eight Wilm's, five
sarcomas, four lymphomas and three hepatoblastomas). hMad2 expression paralleled overall histone H3 phosphoserine 10 expression
in most tumor types. Low hMad2 score tumors (0-3.5) exhibited lower complete response (66.67%) and a higher recurrence/progressive
disease (33.33%) than high score tumors (4-6) (78.57% and 21.43 %), consistent with predicted hMad2 function and reports in
adult tumors. Histone H3 serine 10 phosphoprotein is useful in mitosis enumeration and hMad2 expression may provide additional
help in neuroblastoma profiling and could be useful when spindle checkpoint specific target drugs are used. |
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ISSN: | 0250-7005 1791-7530 |