Validation of the MycoAlign system for Mycobacterium spp. identification

Abstract Validation of the MycoAlign assay, a newly developed Mycobacterium spp. identification system based on internal transcribed spacer-1 sequencing, was performed using 50 acid-fast bacilli (AFB)–positive clinical laboratory specimens. Forty-three (86%) diagnostic-level results were obtained, i...

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Bibliographic Details
Published in:Diagnostic microbiology and infectious disease 2007-09, Vol.59 (1), p.105-108
Main Authors: Olsen, Randall J, Cernoch, Patricia A, Austin, Celest M, Graviss, Edward A, Farkas, Daniel H, Land, Geoffrey A
Format: Article
Language:English
Subjects:
16S
Bacterial Proteins - genetics
Bacteriology
Biological and medical sciences
Chaperonin 60
Chaperonins - genetics
Fundamental and applied biological sciences. Psychology
Genotype
hsp65
Humans
Infectious Disease
Internal Medicine
Internal transcribed region
ITS-1
Microbiology
Miscellaneous
Molecular diagnostics
Molecular Sequence Data
MycoAlign
Mycobacteria
Mycobacterium
Mycobacterium - classification
Mycobacterium - genetics
PCR
Reagent Kits, Diagnostic
RNA, Ribosomal, 16S - genetics
RNA, Ribosomal, 23S - genetics
Sequencing
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Summary:Abstract Validation of the MycoAlign assay, a newly developed Mycobacterium spp. identification system based on internal transcribed spacer-1 sequencing, was performed using 50 acid-fast bacilli (AFB)–positive clinical laboratory specimens. Forty-three (86%) diagnostic-level results were obtained, including 38 Mycobacterium spp. and 5 other AFB-positive genera. Three isolates (6%) had suboptimal identity scores with high probability (81–87% identity score). Four (8%) mixed-pattern results were obtained. Forty-five (90%) observations were concordant with the species identification by standard methods, including all controls.
ISSN:0732-8893
1879-0070
DOI:10.1016/j.diagmicrobio.2007.04.012