Molecular epidemiological analyses of Japanese encephalitis virus isolates from swine in Japan from 2002 to 2004

To characterize Japanese encephalitis virus (JEV) strains recently prevalent in Japan, JEV surveillance was performed in pigs from 2002 to 2004. Eleven new JEV isolates were obtained and compared with previous isolates from Japan and other Asian countries. All of the isolates were classified into ge...

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Bibliographic Details
Published in:Journal of general virology 2007-10, Vol.88 (10), p.2762-2768
Main Authors: Nerome, R, Tajima, S, Takasaki, T, Yoshida, T, Kotaki, A, Lim, C.K, Ito, M, Sugiyama, A, Yamauchi, A, Tano, T
Format: Article
Language:English
Subjects:
animal models
Animals
antiserum
Arthropods - virology
Asia - epidemiology
Biological and medical sciences
disease surveillance
Encephalitis Virus, Japanese - genetics
Encephalitis Virus, Japanese - isolation & purification
Encephalitis Virus, Japanese - pathogenicity
Encephalitis, Japanese - epidemiology
Encephalitis, Japanese - transmission
Encephalitis, Japanese - veterinary
Fundamental and applied biological sciences. Psychology
genes
genotype
Japan - epidemiology
Japanese encephalitis virus
mice
microbial genetics
Microbiology
Miscellaneous
molecular epidemiology
Molecular Sequence Data
nucleotide sequences
serotypes
strain differences
strains
swine
Swine - virology
swine diseases
Swine Diseases - epidemiology
Swine Diseases - transmission
Swine Diseases - virology
viral diseases of animals and humans
Virology
Virulence
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Description
Summary:To characterize Japanese encephalitis virus (JEV) strains recently prevalent in Japan, JEV surveillance was performed in pigs from 2002 to 2004. Eleven new JEV isolates were obtained and compared with previous isolates from Japan and other Asian countries. All of the isolates were classified into genotype 1 by nucleotide sequence analysis of the E gene. Two new isolates with different levels of neurovirulence and neuroinvasiveness, but with only one nucleotide difference in the E gene, Sw/Mie/34/2004 and Sw/Mie/40/2004, were isolated at the same farm on the same day. Sw/Mie/40/2004 displayed higher neurovirulence and neuroinvasiveness in mice than the other four new isolates. Another new isolate, Sw/Hiroshima/25/2002, was neutralized by antiserum to Beijing-1 at a level similar to the homologous Beijing-1 strain, whilst seven other new isolates were neutralized at 10-fold-lower titres. However, there were no amino acid differences in the E protein among these eight isolates. The present study indicated that the 11 new JEV isolates were genetically similar, but biologically and serologically heterogeneous.
ISSN:0022-1317
1465-2099
DOI:10.1099/vir.0.82941-0