Use of biochemical markers of osteoarthritis to investigate the potential disease-modifying effect of tibial plateau levelling osteotomy

To evaluate the hypothesis that the concentration of the 1/20/5D4 epitope of keratan sulphate, cartilage oligomeric matrix protein and total sulphated glycosaminoglycans in synovial fluids from dogs with cranial cruciate ligament disease would be affected by tibial plateau levelling osteotomy. In ad...

Full description

Saved in:
Bibliographic Details
Published in:Journal of small animal practice 2006-12, Vol.47 (12), p.708-714
Main Authors: Girling, S.L, Bell, S.C, Whitelock, R.G, Rayward, R.M, Thomson, D.G, Carter, S.C, Vaughan-Thomas, A, Innes, J.F
Format: Article
Language:English
Subjects:
Animals
Anterior Cruciate Ligament - surgery
Anterior Cruciate Ligament Injuries
arthrotomy
Biomarkers
blood chemistry
cartilage
cartilage metabolism
cranial cruciate ligament disease
dog diseases
Dog Diseases - metabolism
Dogs
Enzyme-Linked Immunosorbent Assay - methods
Enzyme-Linked Immunosorbent Assay - veterinary
Epitopes - analysis
Female
Glycosaminoglycans - analysis
Glycosaminoglycans - immunology
Glycosaminoglycans - metabolism
joint diseases
Keratan Sulfate - analysis
Keratan Sulfate - immunology
Keratan Sulfate - metabolism
Male
osteoarthritis
Osteoarthritis - metabolism
Osteoarthritis - veterinary
osteotomy
Osteotomy - veterinary
pets
Prospective Studies
Rupture
surgery
synovial fluid
Synovial Fluid - chemistry
Synovial Fluid - immunology
tibia
Tibia - surgery
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:To evaluate the hypothesis that the concentration of the 1/20/5D4 epitope of keratan sulphate, cartilage oligomeric matrix protein and total sulphated glycosaminoglycans in synovial fluids from dogs with cranial cruciate ligament disease would be affected by tibial plateau levelling osteotomy. In addition, to evaluate the hypothesis that medial meniscal release or meniscal injury would alter the expression of these candidate biomarkers. Forty-one dogs with naturally occurring cranial cruciate ligament disease were recruited prospectively. Synovial fluids were collected from the index joint before surgery and six weeks and six months postsurgery. Following tibial plateau levelling osteotomy, synovial fluids were assayed for 1/20/5D4 epitope of keratan sulphate and cartilage oligomeric matrix protein concentration using an inhibition ELISA and for sulphated glycosaminoglycans using a direct dye-binding assay. The sulphated glycosaminoglycans ratio did not change significantly during the study. Medial meniscal injury at entry was associated with lower concentrations of synovial fluid cartilage oligomeric matrix protein (P
ISSN:0022-4510
1748-5827
DOI:10.1111/j.1748-5827.2006.00150.x