Pretreatment of hypertonic saline can increase endogenous interleukin 10 release to attenuate hepatic ischemia reperfusion injury

Ischemia-reperfusion (I/R) injury of the liver occurs in many clinical cases. Many steps are associated with hepatic I/R injury, including the release of many inflammatory molecules and infiltration of neutrophils into the liver. Recent studies revealed that hypertonic saline (HTS) has a strong anti...

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Bibliographic Details
Published in:Digestive diseases and sciences 2006-12, Vol.51 (12), p.2257-2263
Main Authors: KE, Qing-Hong, ZHENG, Shu-Sen, LIANG, Ting-Bo, XIE, Hai-Yang, XIA, Wei-Liang
Format: Article
Language:English
Subjects:
Alanine Transaminase - blood
Animals
Biological and medical sciences
Cardiology. Vascular system
Cytokines
Interleukin-10 - metabolism
Ischemia
Liver
Liver - drug effects
Liver - pathology
Liver Diseases - enzymology
Liver Diseases - metabolism
Liver Diseases - pathology
Male
Medical sciences
Neutrophils
Peroxidase - metabolism
Rats
Rats, Sprague-Dawley
Reperfusion Injury - metabolism
Reperfusion Injury - pathology
Reperfusion Injury - prevention & control
Saline Solution, Hypertonic - pharmacology
STAT3 Transcription Factor - metabolism
Tumor Necrosis Factor-alpha - blood
Tumor necrosis factor-TNF
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Summary:Ischemia-reperfusion (I/R) injury of the liver occurs in many clinical cases. Many steps are associated with hepatic I/R injury, including the release of many inflammatory molecules and infiltration of neutrophils into the liver. Recent studies revealed that hypertonic saline (HTS) has a strong anti-inflammatory effect and can inhibit a varity of neutrophil functions. So pretreatment with HTS may attenuate the liver injury associated with I/R. In this study, rats were divided into three groups: the sham group (S group), hepatic I/R group (I/R group), and HTS pretreatment group (HTS group). Serum ALT and myeloperoxidase (MPO) activity were determined. Serum tumor necrosis factor alpha (TNF-alpha) and interleukin 10 (IL-10) were determined by enzyme-linked immunosorbent assay (ELISA). Reverse transcription polymerase chain reaction analysis was used to assess the mRNA expressions of TNF-alpha and IL-10. Protein expressions of TNF-alpha, IL-10, STAT3, and phosphorylated STAT3 were analyzed by Western blot. Results showed that HTS pretreatment can augment the release of endogenous IL-10 by activating STAT3 in the process of hepatic I/R injury. Serum ALT levels, MPO activity in liver, generation of TNF-alpha, and infiltration of neutrophils in liver were inhibited in the HTS group. So we concluded that HTS pretreatment attenuates hepatic I/R injury by increasing the release of endogenous IL-10.
ISSN:0163-2116
1573-2568
DOI:10.1007/s10620-006-9135-6