Comparative proteomics of extracellular proteins in vitro and in planta from the pathogenic fungus Fusarium graminearum

High-throughput MS/MS was used to identify proteins secreted by Fusarium graminearum (Gibberella zeae) during growth on 13 media in vitro and in planta during infection of wheat heads. In vitro secreted proteins were collected from the culture filtrates, and in planta proteins were collected by vacu...

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Bibliographic Details
Published in:Proteomics (Weinheim) 2007-09, Vol.7 (17), p.3171-3183
Main Authors: Paper, Janet M, Scott-Craig, John S, Adhikari, Neil D, Cuomo, Christina A, Walton, Jonathan D
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Chromosome Mapping
Chromosomes, Plant
Fundamental and applied biological sciences. Psychology
Fungal Proteins - analysis
Fusarium - chemistry
Fusarium - genetics
Fusarium - growth & development
Fusarium - pathogenicity
Fusarium head blight
Genes, Fungal
In Vitro Techniques
Mass Spectrometry
Miscellaneous
Models, Biological
Plant Diseases - microbiology
Plant Proteins - analysis
Proteins
Proteome - analysis
Proteomics - methods
Scab
Triticum
Triticum - microbiology
Trypsin - pharmacology
Wheat
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Summary:High-throughput MS/MS was used to identify proteins secreted by Fusarium graminearum (Gibberella zeae) during growth on 13 media in vitro and in planta during infection of wheat heads. In vitro secreted proteins were collected from the culture filtrates, and in planta proteins were collected by vacuum infiltration. A total of 289 proteins (229 in vitro and 120 in planta) were identified with high statistical confidence. Forty-nine of the in planta proteins were not found in any of the in vitro conditions. The majority (91-100%) of the in vitro proteins had predicted signal peptides, but only 56% of the in planta proteins. At least 13 of the nonsecreted proteins found only in planta were single-copy housekeeping enzymes, including enolase, triose phosphate isomerase, phosphoglucomutase, calmodulin, aconitase, and malate dehydrogenase. The presence of these proteins in the in planta but not in vitro secretome might indicate that significant fungal lysis occurs during pathogenesis. On the other hand, several of the proteins lacking signal peptides that were found in planta have been reported to be potent immunogens secreted by animal pathogenic fungi, and therefore could be important in the interaction between F. graminearum and its host plants.
ISSN:1615-9853
1615-9861
DOI:10.1002/pmic.200700184