Nitration of γ-tocopherol in plant tissues

Nitration of γ-tocopherol has been suggested to be an important mechanism for the regulation and detoxification of reactive nitrogen oxide species in animal tissues. To investigate whether this reaction does also occur in plants, reversed phase high-performance liquid chromatography (HPLC) and mass...

Full description

Saved in:
Bibliographic Details
Published in:Planta 2007-10, Vol.226 (5), p.1311-1322
Main Authors: Desel, Christine, Hubbermann, Eva Maria, Schwarz, Karin, Krupinska, Karin
Format: Article
Language:English
Subjects:
5-NγT
Arabidopsis - genetics
Arabidopsis - metabolism
Arabidopsis thaliana
Biological and medical sciences
Cell biochemistry
Cell physiology
Chromatography, High Pressure Liquid
Fundamental and applied biological sciences. Psychology
gamma-Tocopherol - metabolism
Germination
LC-MS
Leaves
Mass Spectrometry
Molecules
Nitrates - metabolism
Nitration
nitric oxide
Nitrogen
Oxides
Plant physiology and development
Plant tissues
Plants
Seeds
Tocopherols
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Nitration of γ-tocopherol has been suggested to be an important mechanism for the regulation and detoxification of reactive nitrogen oxide species in animal tissues. To investigate whether this reaction does also occur in plants, reversed phase high-performance liquid chromatography (HPLC) and mass spectrometry (LC-MS) were used for analysis of 5-nitro-γ-tocopherol (5-NγT) in leaves and seeds. 5-nitro-γ-tocopherol (5-NγT) could be detected in an in vitro system where it was most likely generated by the reaction of γ-tocopherol with a nitric oxide radical. In vivo 5-NγT was identified in leaves of the Arabidopsis mutant line (vte4), which has insertion in the gene encoding γ-tocopherol methyltransferase and consequently lacks α-tocopherol and accumulates high levels of γ-tocopherol. Quantification of NOx in leaves revealed that the vte4 mutant in comparison to wild type and the mutant vte1, which does not contain any tocopherol, has a reduced NOx concentration. The level of 5-NγT in leaves of the vte4 mutant was shown to depend on the developmental stage and on the duration of light exposure. 5-NγT was also detectable in germinating seeds of Brassica napus, Nicotiana tabacum and Arabidopsis thaliana. These seeds have in common high γ-tocopherol contents. The rate of germination at two days after imbibition inversely correlated with the γ-tocopherol content of the seeds. The result suggests that γ-tocopherol or its respective derivative, 5-NγT, may prolong early development by reducing the level of NOx.
ISSN:0032-0935
1432-2048
DOI:10.1007/s00425-007-0552-9