Fabrication and characterization of semicircular detection electrodes for contactless conductivity detector - CE microchips

This study uses simple and reliable microfabrication techniques to fabricate CE biochips, integrating a novel contactless conductivity detector in a miniaturized detection system in a microfluidic biochip. The off‐channel electrodes are deposited around side channels by Au sputtering and patterned u...

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Bibliographic Details
Published in:Electrophoresis 2006-12, Vol.27 (24), p.5043-5050
Main Authors: Lee, Chia-Yen, Chen, C. M., Chang, Guan-Liang, Lin, Che-Hsin, Fu, Lung-Ming
Format: Article
Language:English
Subjects:
Conductometry - methods
Electric Conductivity
Electrophoresis, Capillary - instrumentation
Electrophoresis, Microchip - instrumentation
Gold - chemistry
Lab-on-a-chip
Microelectrodes
Microfabrication
Mineral Waters - analysis
Rhodamines - analysis
Semicircular electrode
Wine - analysis
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Summary:This study uses simple and reliable microfabrication techniques to fabricate CE biochips, integrating a novel contactless conductivity detector in a miniaturized detection system in a microfluidic biochip. The off‐channel electrodes are deposited around side channels by Au sputtering and patterned using a standard “lift‐off” process. A vacuum fusion bonding process is employed to seal the lower substrate containing the microchannels and the electrodes to an upper glass cover plate. The variations in the capacitance between the semicircular detection electrodes in the side channels are measured as different samples and ions pass through the detection region of the CE separation channel. Samples of Rhodamine B, commercial sports drinks, mineral waters, and a red wine, respectively, are mixed in different buffer solutions, separated, and successfully detected using the developed device. The semicircular detection electrodes for the contactless conductivity detector have microscale dimensions and provide a valuable contribution to the realization of the lab‐on‐a‐chip concept.
ISSN:0173-0835
1522-2683
DOI:10.1002/elps.200600113