Isolation and comparative analysis of the wheat TaPT2 promoter: identification in silico of new putative regulatory motifs conserved between monocots and dicots

Phosphorus deficiency is one of the major nutrient stresses affecting plant growth. Plants respond to phosphate (Pi) deficiency through multiple strategies, including the synthesis of high-affinity Pi transporters. In this study, the expression pattern of one putative wheat high-affinity phosphate t...

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Bibliographic Details
Published in:Journal of experimental botany 2007-01, Vol.58 (10), p.2573-2582
Main Authors: Tittarelli, A, Milla, L, Vargas, F, Morales, A, Neupert, C, Meisel, LA, Salvo-G, H, Peñaloza, E, Muñoz, G, Corcuera, LJ, Silva, H
Format: Article
Language:English
Subjects:
Arabidopsis - genetics
Arabidopsis thaliana
Base Sequence
Biology
Computational Biology
Conserved Sequence
Gene Expression Regulation, Plant
Genes
Glucuronidase - analysis
high affinity phosphate transporter
Hordeum vulgare
Molecular Sequence Data
Oryza sativa
Phosphate transport proteins
Phosphate Transport Proteins - chemistry
Phosphate Transport Proteins - genetics
Phosphates
Phosphates - metabolism
Plant Proteins - chemistry
Plant Proteins - genetics
Plant roots
Plant Roots - genetics
Plant Roots - metabolism
Plants
Plants, Genetically Modified - metabolism
promoter
Promoter regions
Promoter Regions, Genetic
Research Papers
Rice
Sequence Alignment
Sequence Analysis, DNA
Sequence Analysis, Protein
Transgenic plants
Triticum - genetics
Triticum aestivum
Wheat
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Summary:Phosphorus deficiency is one of the major nutrient stresses affecting plant growth. Plants respond to phosphate (Pi) deficiency through multiple strategies, including the synthesis of high-affinity Pi transporters. In this study, the expression pattern of one putative wheat high-affinity phosphate transporter, TaPT2, was examined in roots and leaves under Pi-deficient conditions. TaPT2 transcript levels increased in roots of Pi-starved plants. A 579 bp fragment of the TaPT2 promoter is sufficient to drive the expression of the GUS reporter gene specifically in roots of Pi-deprived wheat. This TaPT2 promoter fragment was also able to drive expression of the GUS reporter gene in transgenic Arabidopsis thaliana, under similar growth conditions. Conserved regions and candidate regulatory motifs were detected by comparing this promoter with Pi transporter promoters from barley, rice, and Arabidopsis. Altogether, these results indicate that there are conserved cis-acting elements and trans-acting factors that enable the TaPT2 promoter to be regulated in a tissue-specific and Pi-dependent fashion in both monocots and dicots.
ISSN:0022-0957
1460-2431
DOI:10.1093/jxb/erm123