In vitro antimicrobial activity of propolis samples from different geographical origins against certain oral pathogens

Propolis is an agent having antimicrobial properties, however, its composition can vary depending on the area where it is collected. In the present study, the antimicrobial activity of five propolis samples, collected from four different regions in Turkey and from Brazil, against nine anaerobic stra...

Full description

Saved in:
Bibliographic Details
Published in:Anaerobe 2007-06, Vol.13 (3), p.140-145
Main Authors: Koru, Ozgur, Toksoy, Fulya, Acikel, Cengiz Han, Tunca, Yasar Meric, Baysallar, Mehmet, Uskudar Guclu, Aylin, Akca, Eralp, Ozkok Tuylu, Aslı, Sorkun, Kadriye, Tanyuksel, Mehmet, Salih, Bekir
Format: Article
Language:English
Subjects:
Actinomyces naeslundii
Anaerobic bacteria
Animals
Anti-Bacterial Agents - chemistry
Anti-Bacterial Agents - pharmacology
Antimicrobial activity
Bees
Flavonoids - isolation & purification
Flavonoids - pharmacology
Fusobacterium nucleatum
Gram-Negative Bacteria - drug effects
Gram-Positive Bacteria - drug effects
Lactobacillus acidophilus
Microbial Sensitivity Tests
Mouth Diseases - microbiology
Oral cavity
Peptostreptococcus anaerobius
Porphyromonas gingivalis
Prevotella
Prevotella melaninogenica
Propolis
Propolis - chemistry
Propolis - pharmacology
Veillonella parvula
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Propolis is an agent having antimicrobial properties, however, its composition can vary depending on the area where it is collected. In the present study, the antimicrobial activity of five propolis samples, collected from four different regions in Turkey and from Brazil, against nine anaerobic strains was evaluated. Ethanol extracts of propolis (EEP) were prepared from propolis samples and we determined minimum inhibitory concentrations (MIC) and minimum bactericidal concentrations (MBC) of EEP on the growth of test microorganisms by using agar dilution method. All strains were susceptible and MIC values ranged from 4 to 512 μg/ml for propolis activity. Propolis from Kazan-Ankara showed most effective MIC values to the studied microorganisms. MBC values of Kazan-Ankara EEP samples were ranged from 8 to 512 μg/ml. Death was observed within 4 h of incubation for Peptostreptococcus anaerobius and micros and Lactobacillus acidophilus and Actinomyces naeslundii, while 8 h for Prevotella oralis and Prevotella melaninogenica and Porphyromonas gingivalis, 12 h for Fusobacterium nucleatum, 16 h for Veillonella parvula. It was shown that propolis samples were more effective against Gram positive anaerobic bacteria than Gram negative ones. The organic chemical compositions of EEPs were determined by high-resolution gas chromatography coupled to mass spectrometry (GC–MS). The main compounds of EEPs were flavonoids such as pinobanksin, quercetin, naringenin, galangine, chrysin and aromatic acids such as cafeic acid. Because of increased antimicrobial resistance, propolis may be kept in mind in the treatment of oral cavity diseases.
ISSN:1075-9964
1095-8274
DOI:10.1016/j.anaerobe.2007.02.001