Presynaptic protein distribution and odour mapping in glomeruli of the olfactory bulb of Xenopus laevis tadpoles

The sensory input layer in the olfactory bulb (OB) is typically organized into spheroidal aggregates of dense neuropil called glomeruli. This characteristic compartmentalization of the synaptic neuropil is a typical feature of primary olfactory centres in vertebrates and most advanced invertebrates....

Full description

Saved in:
Bibliographic Details
Published in:The European journal of neuroscience 2007-08, Vol.26 (4), p.925-934
Main Authors: Manzini, Ivan, Heermann, Stephan, Czesnik, Dirk, Brase, Christoph, Schild, Detlev, Rössler, Wolfgang
Format: Article
Language:English
Subjects:
amino acids
Amino Acids - pharmacology
Animals
Axons - physiology
Calcium - metabolism
calcium imaging
Cell Membrane - physiology
Colforsin - pharmacology
forskolin
immunocytochemistry
Immunohistochemistry
Larva - physiology
Lysine - analogs & derivatives
Lysine - pharmacology
Microscopy, Confocal
Nerve Tissue Proteins - metabolism
Olfactory Bulb - anatomy & histology
Olfactory Bulb - physiology
presynaptic proteins
Receptors, Presynaptic - metabolism
Smell - physiology
Xenopus laevis
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The sensory input layer in the olfactory bulb (OB) is typically organized into spheroidal aggregates of dense neuropil called glomeruli. This characteristic compartmentalization of the synaptic neuropil is a typical feature of primary olfactory centres in vertebrates and most advanced invertebrates. In the present work we mapped the location of presynaptic sites in glomeruli across the OB using antibodies to presynaptic vesicle proteins and presynaptic membrane proteins in combination with confocal microscopy. In addition the responses of glomeruli upon mucosal application of amino acid‐odorants and forskolin were monitored using functional calcium imaging. We first describe the spatial distribution of glomeruli across the main olfactory bulb (MOB) in premetamorphic Xenopus laevis. Second, we show that the heterogeneous organization of glomeruli along the dorsoventral and mediolateral axes of the MOB is associated with a differential distribution of synaptic vesicle proteins. While antibodies to synaptophysin, syntaxin and SNAP‐25 uniformly labelled glomeruli in the whole MOB, intense synaptotagmin staining was present only in glomeruli in the lateral, and to a lesser extent in the intermediate, part of the OB. Interestingly, amino acid‐responsive glomeruli were always located in the lateral part of the OB, and glomeruli activated by mucosal forskolin application were exclusively located in the medial part of the OB. This correlation between odour mapping and presynaptic protein distribution is an additional hint on the existence of different subsystems within the main olfactory system in larval Xenopus laevis.
ISSN:0953-816X
1460-9568
DOI:10.1111/j.1460-9568.2007.05731.x