Visualization of hypoxia in microscopic tumors by immunofluorescent microscopy

Tumor hypoxia is commonly observed in primary solid malignancies but the hypoxic status of subclinical micrometastatic disease is largely unknown. The distribution of hypoxia in microscopic tumors was studied in animal models of disseminated peritoneal disease and intradermal (i.d.) growing tumors....

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Published in:Cancer research (Chicago, Ill.) Ill.), 2007-08, Vol.67 (16), p.7646-7653
Main Authors: LI, Xiao-Feng, CARLIN, Sean, URANO, Muneyasu, RUSSELL, James, LING, C. Clifton, O'DONOGHUE, Joseph A
Format: Article
Language:English
Subjects:
Animals
Antineoplastic agents
Ascites - metabolism
Ascites - pathology
Biological and medical sciences
Carbon Dioxide - pharmacology
Cell Hypoxia - physiology
Cell Line, Tumor
Colonic Neoplasms - metabolism
Colonic Neoplasms - pathology
Female
HT29 Cells
Humans
Medical sciences
Mice
Mice, Nude
Microscopy, Fluorescence
Neoplasm Transplantation
Oxygen - metabolism
Oxygen - pharmacology
Peritoneal Neoplasms - metabolism
Peritoneal Neoplasms - pathology
Pharmacology. Drug treatments
Transplantation, Heterologous
Tumors
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Summary:Tumor hypoxia is commonly observed in primary solid malignancies but the hypoxic status of subclinical micrometastatic disease is largely unknown. The distribution of hypoxia in microscopic tumors was studied in animal models of disseminated peritoneal disease and intradermal (i.d.) growing tumors. Tumors derived from human colorectal adenocarcinoma cell lines HT29 and HCT-8 ranged in size from a few hundred microns to several millimeters in diameter. Hypoxia was detected by immunofluorescent visualization of pimonidazole and the hypoxia-regulated protein carbonic anhydrase 9. Tumor blood perfusion, cellular proliferation, and vascularity were visualized using Hoechst 33342, bromodeoxyuridine, and CD31 staining, respectively. In general, tumors of
ISSN:0008-5472
1538-7445
DOI:10.1158/0008-5472.CAN-06-4353