Expression of mRNA MMP-7 and mRNA TIMP-1 in Non-small Cell Lung Cancer

Background: Destruction of the extracellular matrix is a necessary precondition for metastasis and invasion of tumour cells. Metalloproteinases (MMPs) are involved in this process, matrilysin being one of them (MMP-7). The results of our pilot study with patients operated on for non-small cell lung...

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Bibliographic Details
Published in:Anticancer research 2007-07, Vol.27 (4C), p.2953-2956
Main Authors: SAFRANEK, J, HOLUBEC, L, TOPOLCAN, O, PESTA, M, KLECKA, J, VODICKA, J, FINEK, J, KORMUNDA, S, PESEK, M
Format: Article
Language:English
Subjects:
Aged
Biological and medical sciences
Carcinoma, Non-Small-Cell Lung - enzymology
Carcinoma, Non-Small-Cell Lung - genetics
Carcinoma, Non-Small-Cell Lung - metabolism
Carcinoma, Non-Small-Cell Lung - pathology
Humans
Lung Neoplasms - enzymology
Lung Neoplasms - genetics
Lung Neoplasms - metabolism
Lung Neoplasms - pathology
Matrix Metalloproteinase 7 - biosynthesis
Matrix Metalloproteinase 7 - genetics
Medical sciences
Middle Aged
Neoplasm Staging
Pilot Projects
Pneumology
RNA, Messenger - biosynthesis
RNA, Messenger - genetics
Tissue Inhibitor of Metalloproteinase-1 - biosynthesis
Tissue Inhibitor of Metalloproteinase-1 - genetics
Tumors
Tumors of the respiratory system and mediastinum
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Summary:Background: Destruction of the extracellular matrix is a necessary precondition for metastasis and invasion of tumour cells. Metalloproteinases (MMPs) are involved in this process, matrilysin being one of them (MMP-7). The results of our pilot study with patients operated on for non-small cell lung carcinoma (NSCLC), with the assessment of MMP-7 and the tissue inhibitor of matrix metalloproteinase (TIMP-1), are presented here. Patients and Methods: The group consisted of 34 patients who had been operated on in the course of 2005. Messenger RNA MMP-7 and TIMP-1 were assessed in 20 cases (58%). Tissue samples were frozen to -70ÆC, total RNA was subsequently isolated and a reverse transcription was performed from it. The quantitative assessment itself was performed using a real-time PCR method. The resulting expression level was determined as the expression ratio of the assessed gene and the housekeeping gene, glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Results: A higher expression of mRNA MMP-7 was found in the NSCLC tissue than in non-tumourous lung tissue. On the other hand, a higher expression of mRNA TIMP-1 in the non-tumourous surrounding lung tissue was demonstrated. The expression of mRNA MMP-7 and TIMP-1 was higher in adenocarcinoma than in the epidermoid form of NSCLC. Conclusion: The value of our results should not be overestimated since we had only a small group of patients and assessed only one of the whole range of metalloproteinases (MMP-7). We consider the assessment and ratio quantification of metallorpoteinases in normal lung and NSCLC to be the first step in a further application of these parameters.
ISSN:0250-7005
1791-7530