Functional Links between the Fusion Peptide-proximal Polar Segment and Membrane-proximal Region of Human Immunodeficiency Virus gp41 in Distinct Phases of Membrane Fusion

The binding of CD4 and chemokine receptors to the gp120 attachment glycoprotein of human immunodeficiency virus triggers refolding of the associated gp41 fusion glycoprotein into a trimer of hairpins with a 6-helix bundle (6HB) core. These events lead to membrane fusion and viral entry. Here, we exa...

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Bibliographic Details
Published in:The Journal of biological chemistry 2007-08, Vol.282 (32), p.23104-23116
Main Authors: Bellamy-McIntyre, Anna K., Lay, Chan-Sien, Baaör, Séverine, Maerz, Anne L., Talbo, Gert H., Drummer, Heidi E., Poumbourios, Pantelis
Format: Article
Language:English
Subjects:
Alanine - chemistry
Amino Acid Sequence
CD4-Positive T-Lymphocytes - virology
Cell Line
Dimerization
Genetic Vectors
Glycoproteins - chemistry
HIV Envelope Protein gp120 - chemistry
HIV Envelope Protein gp41 - chemistry
Human immunodeficiency virus
Humans
Leucine - chemistry
Molecular Sequence Data
Protein Structure, Tertiary
Recombinant Fusion Proteins - chemistry
Sequence Homology, Amino Acid
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Summary:The binding of CD4 and chemokine receptors to the gp120 attachment glycoprotein of human immunodeficiency virus triggers refolding of the associated gp41 fusion glycoprotein into a trimer of hairpins with a 6-helix bundle (6HB) core. These events lead to membrane fusion and viral entry. Here, we examined the functions of the fusion peptide-proximal polar segment and membrane-proximal Trp-rich region (MPR), which are exterior to the 6HB. Alanine substitution of Trp666, Trp672, Phe673, and Ile675 in the MPR reduced entry by up to 120-fold without affecting gp120-gp41 association or cell-cell fusion. The L537A polar segment mutation led to the loss of gp120 from the gp120-gp41 complex, reduced entry by ∼10-fold, but did not affect cell-cell fusion. Simultaneous Ala substitution of Leu537 with Trp666, Trp672, Phe673, or Ile675 abolished entry with 50–80% reductions in cell-cell fusion. gp120-gp41 complexes of fusion-defective double mutants were resistant to soluble CD4-induced shedding of gp120, suggesting that their ability to undergo receptor-induced conformational changes was compromised. Consistent with this idea, a representative mutation, L537A/W666A, led to an ∼80% reduction in lipophilic fluorescent dye transfer between gp120-gp41-expressing cells and receptor-expressing targets, indicating a block prior to the lipid-mixing phase. The L537A/W666A double mutation increased the chymotrypsin sensitivity of the polar segment in a trimer of hairpins model, comprising the 6HB core, the polar segment, and MPR linked N-terminally to maltose-binding protein. The data indicate that the polar segment and MPR of gp41 act synergistically in forming a fusion-competent gp120-gp41 complex and in stabilizing the membrane-interactive end of the trimer of hairpins.
ISSN:0021-9258
1083-351X
DOI:10.1074/jbc.M703485200