Identification and preliminary function study of Xenopus laevis DRR1 gene

Xenopus laevis has recently been determined as a novel study platform of gene function. In this study, we cloned Xenopus DRR1 (xDRR1), which is homologous to human down-regulated in renal carcinoma (DRR1) gene. Bioinformatics analysis for DRR1 indicated that xDRR1 shared 74% identity with human DRR1...

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Published in:Biochemical and biophysical research communications 2007-09, Vol.361 (1), p.74-78
Main Authors: Zhao, Xin-Yu, Liang, Shu-Fang, Yao, Shao-Hua, Ma, Fan-Xin, Hu, Zhong-Guo, Yan, Fei, Yuan, Zhu, Ruan, Xu-Zhi, Yang, Han-Shuo, Zhou, Qin, Wei, Yu-Quan
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Cloning, Molecular
Embryo development
Embryonic Development
Eukaryotic expression
Genes, Tumor Suppressor
Growth Inhibitors - chemistry
Growth Inhibitors - genetics
Growth Inhibitors - physiology
Humans
Molecular Sequence Data
Nuclear Proteins - chemistry
Nuclear Proteins - genetics
Nuclear Proteins - metabolism
Nuclear Proteins - physiology
Sequence Homology, Amino Acid
Xenopus down-regulated in renal cell carcinoma (xDRR1)
Xenopus laevis
Xenopus Proteins - chemistry
Xenopus Proteins - genetics
Xenopus Proteins - physiology
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Summary:Xenopus laevis has recently been determined as a novel study platform of gene function. In this study, we cloned Xenopus DRR1 (xDRR1), which is homologous to human down-regulated in renal carcinoma (DRR1) gene. Bioinformatics analysis for DRR1 indicated that xDRR1 shared 74% identity with human DRR1 and 66% with mouse DRR1, and the phlogenetic tree of DRR1 protein was summarized. The xDRR1 gene locates in nuclei determined by transfecting A549 cells with the recombinant plasmid pEGFP-N1/xDRR1. RT-PCR analysis revealed that xDRR1 gene was expressed in all stages of early embryo development and all kinds of detected tissues, and whole-mount in situ hybridization showed xDRR1 was mainly present along ectoderm and mesoderm. Furthermore, xDRR1 expression could suppress A549 cell growth by transfecting with plasmid pcDNA3.1(+)/xDRR1. xDRR1 probably plays important roles involving in cell growth regulation and Xenopus embryo development.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2007.06.158