Immunohistochemical properties of ocular adenomas, adenocarcinomas and medulloepitheliomas

Ocular medulloepitheliomas, adenomas and adenocarcinomas share a common phenotype and originate from the optic cup neuroectoderm. This can make it very difficult to differentiate between these tumors histopathologically. Therefore, this study focused on identifying a combination of immunologic marke...

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Bibliographic Details
Published in:Veterinary ophthalmology 2006-11, Vol.9 (6), p.387-394
Main Authors: Klosterman, E, Colitz, C.M.H, Chandler, H.L, Kusewitt, D.F, Saville, W.J.A, Dubielzig, R.R
Format: Article
Language:English
Subjects:
adenocarcinoma
Adenocarcinoma - diagnosis
Adenocarcinoma - immunology
Adenocarcinoma - veterinary
adenoma
Adenoma - diagnosis
Adenoma - immunology
Adenoma - veterinary
animal diseases
Animals
Antibodies
antibody detection
biomarkers
Biomarkers, Tumor - immunology
canine
ciliary body epithelium
cytokeratins
differential staining
disease detection
disease diagnosis
Dog Diseases - diagnosis
Dog Diseases - immunology
Dogs
epithelium
eye diseases
Eye Neoplasms - diagnosis
Eye Neoplasms - immunology
Eye Neoplasms - veterinary
eyes
Female
immunohistochemistry
Immunohistochemistry - veterinary
keratin
Male
medulloepithelioma
neoplasms
Neuroectodermal Tumors, Primitive - diagnosis
Neuroectodermal Tumors, Primitive - immunology
Neuroectodermal Tumors, Primitive - veterinary
Predictive Value of Tests
RNA-directed DNA polymerase
telomerase
telomerase reverse transcriptase
telomerase reverse transcriptase (TERT)
veterinary medicine
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Summary:Ocular medulloepitheliomas, adenomas and adenocarcinomas share a common phenotype and originate from the optic cup neuroectoderm. This can make it very difficult to differentiate between these tumors histopathologically. Therefore, this study focused on identifying a combination of immunologic markers that might be used in the diagnosis of these tumors. These markers included AE1/AE3, CK7, CK20, and telomerase reverse transcriptase (TERT). Routine immunohistochemical staining was performed on 27 whole globes diagnosed with one of these tumors. The tumors that immunostained for TERT showed increasing immunoreactivity as the tumor types increased in aggressiveness. None of the tumor types were immunopositive for CK7. CK20 immunostaining was found in the adenomas but not in the adenocarcinomas or medulloepitheliomas. AE1/AE3 expression was present more consistently in the adenocarcinomas and less frequently in the adenomas. AE1/AE3 expression was present in only one of six medulloepitheliomas. Furthermore, CK20 and TERT showed inverse expression patterns, i.e. TERT increased in expression and CK20 decreased in expression with increasing aggressiveness. These results may be important diagnostic and prognostic indicators for these tumors.
ISSN:1463-5216
1463-5224
DOI:10.1111/j.1463-5224.2006.00478.x