Distinct immunohistochemical localization in Kuru plaques using novel anti-prion protein antibodies

ABSTRACT By immunizing Prnp‐knockout mice with synthetic polypeptides, a panel of mAbs directed to bovine PrPC was obtained. The mAb panel was characterized by the ELISA method, where synthetic polypeptides were used for epitope mapping. Different reactivity patterns were identified. The ability of...

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Bibliographic Details
Published in:Microbiology and immunology 2008-01, Vol.52 (1), p.25-29
Main Authors: Hosokawa, Tomoko, Ono, Fumiko, Tsuchiya, Kotaro, Sato, Ichiro, Takeyama, Natsumi, Ueda, Susumu, Zanusso, Gianluigi, Takahashi, Hidehiro, Sata, Tetsutaro, Sakudo, Akikazu, Suguira, Katsuaki, Baj, Andreina, Toniolo, Antonio, Yoshikawa, Yasuhiro, Onodera, Takashi
Format: Article
Language:English
Subjects:
Animals
Antibodies - immunology
Antibodies - isolation & purification
Antibodies, Monoclonal - immunology
Antibodies, Monoclonal - isolation & purification
bovine spongiform encephalopathy
Brain - pathology
Cattle
Creutzfeldt-Jakob disease
Epitope Mapping
Humans
Kuru - pathology
Mice
Mice, Knockout
prion
Prions - analysis
Prions - immunology
Saimiri
scrapie
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Summary:ABSTRACT By immunizing Prnp‐knockout mice with synthetic polypeptides, a panel of mAbs directed to bovine PrPC was obtained. The mAb panel was characterized by the ELISA method, where synthetic polypeptides were used for epitope mapping. Different reactivity patterns were identified. The ability of these mAbs to detect abnormal PrPSc in CJD cases was studied by immunohistochemistry. All mAbs were tested for PrPSc in murine, bovine, monkey and human brain tissues. Three mAbs recognized the fragmented PrP epitope in our ELISA. Antibody 1D12 was strongly reactive to ovine and squirrel monkey tissues infected with a scrapie agent, although non‐reactive to scrapie‐infected mouse tissues. Antibody 2D8 was clearly reactive to type‐2 but not type‐1 CJD human tissues. Of particular interest was the reactivity of mAb 6C4 with the inner structure of Kuru plaques (peripheral pattern) in a type‐2 CJD case and mAb T2, 1D12, 2B11, 2D8, 4B5 and 6G3‐2 with the central area (central pattern). The fact that different anti‐PrP mAbs possess distinct staining properties suggests that the PrPc to PrPSc conversion might involve a multiple‐step process.
ISSN:0385-5600
1348-0421
DOI:10.1111/j.1348-0421.2008.00007.x