Fast determination of conditions for maximum dynamic capacity in cation-exchange chromatography of human monoclonal antibodies

Dynamic binding capacity (DBC) measurements of cation-exchange resins were performed with two human monoclonal antibodies. DBC showed a pH dependent maximum, which was shifted to lower pH values with increasing buffer concentrations and increasing salting-out effect of the buffer anion according to...

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Bibliographic Details
Published in:Journal of Chromatography A 2007-08, Vol.1161 (1), p.29-35
Main Authors: Faude, Alexander, Zacher, Dörthe, Müller, Egbert, Böttinger, Heiner
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Antibodies, Monoclonal - isolation & purification
Biological and medical sciences
Cation Exchange Resins
Cation-exchange chromatography
Chromatography, Ion Exchange - methods
Dynamic binding capacity
Fundamental and applied biological sciences. Psychology
Glycoproteins
Hofmeister series
Human monoclonal antibodies
Humans
Hydrogen-Ion Concentration
Proteins
Purification process development
Zeta potential
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Summary:Dynamic binding capacity (DBC) measurements of cation-exchange resins were performed with two human monoclonal antibodies. DBC showed a pH dependent maximum, which was shifted to lower pH values with increasing buffer concentrations and increasing salting-out effect of the buffer anion according to the Hofmeister series. As this downshift correlates well with zeta potential values, a measurement of the latter allows the determination of the pH value for maximum DBC under a given set of conditions. Thus, the use of zeta potential values can accelerate the purification process development and helps to understand the protein adsorption mechanism.
ISSN:0021-9673
DOI:10.1016/j.chroma.2007.03.114