Extrafollicular Activation of Lymph Node B Cells by Antigen-Bearing Dendritic Cells

In contrast to naïve T cells that recognize short antigen-derived peptides displayed by specialized antigen-presenting cells, immunoglobulin receptors of B lymphocytes primarily recognize intact proteins. How and where within a lymph node such unprocessed antigens become available for naïve B cell r...

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Bibliographic Details
Published in:	Science (American Association for the Advancement of Science) 2006-06, Vol.312 (5780), p.1672-1676
Main Authors:	Qi, Hai, Egen, Jackson G, Huang, Alex Y.C, Germain, Ronald N
Format:	Article
Language:	eng
Subjects:	Analysis of the immune response. Humoral and cellular immunity Animals Antibodies Antigens Antigens - immunology B lymphocytes B-Lymphocytes - cytology B-Lymphocytes - immunology Biological and medical sciences Calcium - metabolism Calcium Signaling Cell interactions Cell Movement Cell Shape Coculture Techniques Dendritic cells Dendritic Cells - immunology Endocytosis Follicles Fundamental and applied biological sciences. Psychology Fundamental immunology Hepatitis E virus Human migration Immunity Immunobiology Immunoglobulins Immunology Lymph nodes Lymph Nodes - cytology Lymph Nodes - immunology Lymphatic system Lymphocyte Activation Lymphocytes Mice Mice, Inbred C57BL Mice, Transgenic Muramidase - immunology Receptors, Antigen, B-Cell - immunology Signal transduction T lymphocytes T-Lymphocytes - immunology
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Summary:	In contrast to naïve T cells that recognize short antigen-derived peptides displayed by specialized antigen-presenting cells, immunoglobulin receptors of B lymphocytes primarily recognize intact proteins. How and where within a lymph node such unprocessed antigens become available for naïve B cell recognition is not clear. We used two-photon intravital imaging to show that, after exiting high-endothelial venules and before entry into lymph node follicles, B cells survey locally concentrated dendritic cells. Engagement of the B cell receptor by the dendritic cell (DC)-associated antigen leads to lymphocyte calcium signaling, migration arrest, antigen acquisition, and extrafollicular accumulation. These findings suggest a possible role for antigen-specific B-DC interactions in promoting T cell-dependent antibody responses in vivo.
ISSN:	0036-8075 1095-9203