Evaluation of Chromocult enterococci agar for the isolation and selective enumeration of Enterococcus spp. in broilers

Aims:  To investigate the productivity and specificity of a new chromogenic enterococci selective medium (Chromocult® enterococci agar) recently developed by Merck. Methods and Results:  The study was carried out comparing Chromocult® enterococci agar with MRS agar (Merck), a basal lactic acid bacte...

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Bibliographic Details
Published in:Letters in applied microbiology 2005-01, Vol.41 (2), p.153-156
Main Authors: Miranda, J.M, Franco, C.M, Vazquez, B.I, Fente, C.A, Barros-Velazquez, J, Cepeda, A
Format: Article
Language:English
Subjects:
Agar
Animals
bacterial contamination
Biological and medical sciences
broiler chickens
Chickens - microbiology
Chromocult
Chromocult enterococci agar
chromogenic media
Colony Count, Microbial
Culture Media
enterococci
Enterococcus
Enterococcus - growth & development
Enterococcus - isolation & purification
evaluation
feces
Feces - microbiology
food contamination
food microbiology
Fundamental and applied biological sciences. Psychology
isolation
Microbiology
poultry
selective media
Sensitivity and Specificity
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Summary:Aims:  To investigate the productivity and specificity of a new chromogenic enterococci selective medium (Chromocult® enterococci agar) recently developed by Merck. Methods and Results:  The study was carried out comparing Chromocult® enterococci agar with MRS agar (Merck), a basal lactic acid bacteria medium in current use. A total of 216 faecal samples from poultry were collected and enterococci populations were counted. Likewise, 100 randomly selected strains were identified for each medium. The differences found between the two media were analysed and discussed. Conclusions:  A good sensitivity of 98% was obtained for Chromocult® agar and all false‐positive isolates obtained were identified as Leuconostoc spp. However significant differences (P 
ISSN:0266-8254
1472-765X
DOI:10.1111/j.1472-765X.2005.01728.x