Endoplasmic reticulum targeted GFP reveals ER organization in tobacco NT-1 cells during cell division

The endoplasmic reticulum (ER) of plant cells undergoes a drastic reorganization during cell division. In tobacco NT-1 cells that stably express a GFP construct targeted to the ER, we have mapped the reorganization of ER that occurs during mitosis and cytokinesis with confocal laser scanning microsc...

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Bibliographic Details
Published in:Plant physiology and biochemistry 2006-02, Vol.44 (2), p.95-105
Main Authors: Gupton, S.L., Collings, D.A., Allen, N.S.
Format: Article
Language:English
Subjects:
Actin microfilaments
Actins - metabolism
Biological and medical sciences
Cell division
Cell kinetics
Cell Line
Cell physiology
Chromosomes, Plant - physiology
Cytokinesis - physiology
Endoplasmic reticulum
Endoplasmic Reticulum - metabolism
Fundamental and applied biological sciences. Psychology
Green fluorescent protein
Green Fluorescent Proteins - metabolism
Interphase - physiology
Microtubules
Nicotiana - cytology
Nicotiana - metabolism
Nuclear envelope
Nuclear Envelope - metabolism
Nuclear invaginations
Plant physiology and development
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Summary:The endoplasmic reticulum (ER) of plant cells undergoes a drastic reorganization during cell division. In tobacco NT-1 cells that stably express a GFP construct targeted to the ER, we have mapped the reorganization of ER that occurs during mitosis and cytokinesis with confocal laser scanning microscopy. During division, the ER and nuclear envelope do not vesiculate. Instead, tubules of ER accumulate around the chromosomes after the nuclear envelope breaks down, with these tubules aligning parallel to the microtubules of the mitotic spindle. In cytokinesis, the phragmoplast is particularly rich in ER, and the transnuclear channels and invaginations present in many interphase cells appear to develop from ER tubules trapped in the developing phragmoplast. Drug studies, using oryzalin and latrunculin to disrupt the microtubules and actin microfilaments, respectively, demonstrate that during division, the arrangement of ER is controlled by microtubules and not by actin, which is the reverse of the situation in interphase cells.
ISSN:0981-9428
1873-2690
DOI:10.1016/j.plaphy.2006.03.003