Butanol Production from Corn Fiber Xylan Using Clostridium acetobutylicum

Acetone, butanol, and ethanol (ABE) were produced from corn fiber arabinoxylan (CFAX) and CFAX sugars (glucose, xylose, galactose, and arabinose) using Clostridium acetobutylicum P260. In mixed sugar (glucose, xylose, galactose, and arabinose) fermentation, the culture preferred glucose and arabinos...

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Bibliographic Details
Published in:Biotechnology progress 2006, Vol.22 (3), p.673-680
Main Authors: Qureshi, N, Li, X.L, Hughes, S, Saha, B.C, Cotta, M.A
Format: Article
Language:English
Subjects:
acetone
alcoholic fermentation
arabinose
Biological and medical sciences
Biotechnology
butanol
Butanols - chemistry
Butanols - metabolism
Clostridium - chemistry
Clostridium - metabolism
Clostridium acetobutylicum
corn
Endo-1,4-beta Xylanases - metabolism
Enzyme Activation - physiology
ethanol
ethanol production
Fermentation
Fundamental and applied biological sciences. Psychology
galactose
glucose
Glucose - chemistry
Glucose - metabolism
plant fibers
Textiles
Time Factors
xylan
Xylans - chemistry
Xylans - metabolism
xylose
Xylose - chemistry
Xylose - metabolism
Zea mays
Zea mays - chemistry
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Summary:Acetone, butanol, and ethanol (ABE) were produced from corn fiber arabinoxylan (CFAX) and CFAX sugars (glucose, xylose, galactose, and arabinose) using Clostridium acetobutylicum P260. In mixed sugar (glucose, xylose, galactose, and arabinose) fermentation, the culture preferred glucose and arabinose over galactose and xylose. Under the experimental conditions, CFAX (60 g/L) was not fermented until either 5 g/L xylose or glucose plus xylanase enzyme were added to support initial growth and fermentation. In this system, C. acetobutylicum produced 9.60 g/L ABE from CFAX and xylose. This experiment resulted in a yield and productivity of 0.41 and 0.20 g/L·h, respectively. In the integrated hydrolysis, fermentation, and recovery process, 60 g/L CFAX and 5 g/L xylose produced 24.67 g/L ABE and resulted in a higher yield (0.44) and a higher productivity (0.47 g/L·h). CFAX was hydrolyzed by xylan‐hydrolyzing enzymes, and ABE were recovered by gas stripping. This investigation demonstrated that integration of hydrolysis of CFAX, fermentation to ABE, and recovery of ABE in a single system is an economically attractive process. It is suggested that the culture be further developed to hydrolyze CFAX and utilize all xylan sugars simultaneously. This would further increase productivity of the reactor.
ISSN:8756-7938
1520-6033
DOI:10.1021/bp050360w