ACIDIC FIBROBLAST GROWTH FACTOR PROMOTES HEPATIC DIFFERENTIATION OF MONKEY EMBRYONIC STEM CELLS

Embryonic stem (ES) cells can replicate indefinitely and differentiate into all cell types, including hepatocytes. Research using primate ES cells is considered to be important for studies of potential cell therapies. Recently, we established cynomolgus monkey ES cells designated as CMK6. The CMK6 c...

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Bibliographic Details
Published in:In vitro cellular & developmental biology. Animal 2006-03, Vol.42 (3), p.83-88
Main Authors: TSUKADA, HIROAKI, TAKADA, TATSUYUKI, SHIOMI, HISANORI, TORII, RYUZO, TANI, TOHRU
Format: Article
Language:English
Subjects:
Albs
Albumins - metabolism
Animals
Biomarkers
Cell Differentiation - physiology
Cell Growth/Differentiation/Apoptosis
Cell Line
Cell Lineage
Cell lines
Cellular differentiation
culture
Cynomolgus
Embryonic stem cells
Fibroblast Growth Factor 1 - metabolism
Hepatocytes
Hepatocytes - cytology
Hepatocytes - physiology
Humans
liver
Macaca fascicularis
Multipotent stem cells
Neural stem cells
Pluripotent stem cells
Pluripotent Stem Cells - cytology
Pluripotent Stem Cells - physiology
primate
Primates
regenerative medicine
Stem cells
Tissue therapy
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Summary:Embryonic stem (ES) cells can replicate indefinitely and differentiate into all cell types, including hepatocytes. Research using primate ES cells is considered to be important for studies of potential cell therapies. Recently, we established cynomolgus monkey ES cells designated as CMK6. The CMK6 cell line is a useful tool for investigating the mechanism of differentiation in primate ES cells and developing cell therapies, because of its biological similarity to human ES cells. To examine whether cynomolgus monkey ES cells differentiate into hepatocytes, CMK6 cells were cultured with or without acidic fibroblast growth factor (aFGF). Evaluation of the hepatic differentiation was performed by analysis of the mRNA expression in early hepatic marker genes using the reverse transcriptase–polymerase chain reaction (RT-PCR). The protein expression of albumin (ALB) was also studied by immunocytochemistry. RT-PCR analyses revealed mRNA expressions of alpha-fetoprotein, transthyretin, and ALB in the presence of aFGF at 3 wk of differentiation, whereas no mRNA expression of these genes was detected in cells without aFGF. The protein expression of ALB in the presence of aFGF at 3 wk of differentiation was also confirmed by immunocytochemistry. However, tyrosine aminotransferase, which is a mature hepatic marker, was not detected in the presence or absence of aFGF at any stage of differentiation. These results suggested that aFGF successfully promoted in vitro differentiation of cynomolgus monkey ES cells to an early hepatic lineage.
ISSN:1071-2690
1543-706X
1543-706X
DOI:10.1290/0506039.1