Effect of 2-deoxy-D-glucose on aminoacids metabolism in rats' cerebral cortex slices

We studied the effect of different concentrations of 2-deoxy-D-glucose on the L-[U-14C]leucine, L-[1-14C]leucine and [1-14C]glycine metabolism in slices of cerebral cortex of 10-day-old rats. 2-deoxy-D-glucose since 0.5 mM concentration has inhibited significantly the protein synthesis from L-[U-14C...

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Published in:Neurochemical research 2006-03, Vol.31 (3), p.417-422
Main Authors: Muller, Alexandre P, Rotta, Liane N, Kawano, Cristina, Leszczinski, Daniel N, Schweigert, Ingrid D, Londero, Lisiane G, Gravina, Fernanda S, da Silveira, Clarice K B, de Souza, Carolina G, Battu, Cíntia E, Gonçalves, Carlos A, de Souza, Diogo O, Perry, Marcos L S
Format: Article
Language:English
Subjects:
Animals
Carbon Dioxide - metabolism
Carbon Radioisotopes
Cerebral Cortex - drug effects
Cerebral Cortex - metabolism
Deoxyglucose - metabolism
Deoxyglucose - pharmacology
Glucose - metabolism
Glycine - metabolism
In Vitro Techniques
Lactic Acid - metabolism
Leucine - metabolism
Lipids - biosynthesis
Phosphorylation
Protein Biosynthesis - drug effects
Rats
Rats, Wistar
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Summary:We studied the effect of different concentrations of 2-deoxy-D-glucose on the L-[U-14C]leucine, L-[1-14C]leucine and [1-14C]glycine metabolism in slices of cerebral cortex of 10-day-old rats. 2-deoxy-D-glucose since 0.5 mM concentration has inhibited significantly the protein synthesis from L-[U-14C]leucine and from [1-14C]glycine in relation to the medium containing only Krebs Ringer bicarbonate. Potassium 8.0 mM in incubation medium did not stimulate the protein synthesis compared to the medium containing 2.7 mM, and at 50 mM diminishes more than 2.5 times the protein synthesis compared to the other concentration. Only at the concentration of 5.0 mM, 2-deoxy-D-glucose inhibited the CO2 production and lipid synthesis from L-[U-14C] leucine. This compound did not inhibit either CO2 production, or lipid synthesis from [1-14C]glycine. Lactate at 10 mM and glucose 5.0 mM did not revert the inhibitory effect of 2-deoxy-D-glucose on the protein synthesis from L-[U-14C]leucine. 2-deoxy-D-glucose at 2.0 mM did not show any effect either on CO2 production, or on lipid synthesis from L-[U-14C]lactate 10 mM and glucose 5.0 mM.
ISSN:0364-3190
1573-6903
DOI:10.1007/s11064-005-9031-5