Montenegro's skin reactions and antibodies against different Leishmania species in dogs from a visceral leishmaniosis endemic area

In this study, humoral (circulating anti- Leishmania antibodies) and cellular (Montenegro's skin test) immune responses of dogs from an endemic area of visceral leishmaniosis were tested using Leishmania chagasi, Leishmania amazonensis and Leishmania braziliensis antigens. The antibody response...

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Bibliographic Details
Published in:Veterinary parasitology 2006-06, Vol.139 (1), p.21-28
Main Authors: Baleeiro, Carolina O., Paranhos-Silva, Moacir, dos Santos, Juliana C., Oliveira, Geraldo G.S., Nascimento, Eliane Goes, de Carvalho, Lain Pontes, dos-Santos, Washington L.C.
Format: Article
Language:English
Subjects:
Analysis of Variance
Animals
Antibodies, Protozoan - blood
antibody detection
antibody formation
Antibody-response
antigens
Antigens, Protozoan - immunology
Brazil - epidemiology
cell-mediated immunity
Dog Diseases - epidemiology
Dog Diseases - parasitology
Dogs
enzyme-linked immunosorbent assay
Enzyme-Linked Immunosorbent Assay - methods
Enzyme-Linked Immunosorbent Assay - veterinary
humoral immunity
immune response
Leishmania - classification
Leishmania - immunology
Leishmania amazonensis
Leishmania braziliensis
Leishmania braziliensis - immunology
Leishmania chagasi
Leishmania donovani
Leishmaniasis
Leishmaniasis - epidemiology
Leishmaniasis - veterinary
Leishmaniasis, Cutaneous - epidemiology
Leishmaniasis, Cutaneous - veterinary
Leishmaniasis, Visceral - epidemiology
Leishmaniasis, Visceral - veterinary
Leishmanin skin test
Phylogeny
Seroepidemiologic Studies
Skin - immunology
skin tests
Skin Tests - veterinary
Species Specificity
visceral leishmaniasis
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Summary:In this study, humoral (circulating anti- Leishmania antibodies) and cellular (Montenegro's skin test) immune responses of dogs from an endemic area of visceral leishmaniosis were tested using Leishmania chagasi, Leishmania amazonensis and Leishmania braziliensis antigens. The antibody response was tested in three animal groups, selected according to their anti- L. chagasi antibody activity, as measured by ELISA in the serum: 19 negative (O.D. below 0.30), seven with undefined (O.D. between 0.40 and 0.70) and 12 positive (O.D. above 1.0) ELISA result. In the group of animals with positive ELISA, the antibody activity against L. chagasi antigens (mean O.D. = 1.31) was significantly higher (ANOVA, P < 0.01) than against L. amazonensis (mean O.D. = 0.88) or L. braziliensis (mean O.D. = 0.87) antigens. The Montenegro's skin test results obtained with L. chagasi and L. braziliensis antigens showed a fair agreement ( κ = 0.309). The same was observed when antigens from L. braziliensis and L. amazonensis were compared ( κ = 0.374), whereas a moderate agreement between the results from tests performed with L. chagasi and L. amazonensis antigens was observed ( κ = 0.530). The induration areas obtained with L. braziliensis antigen were smaller than those obtained with the other antigens. The data presented herein indicate that the use of antigens from different Leishmania species may interfere with the results of the immunological tests performed in dogs in an endemic area of visceral leishmaniosis.
ISSN:0304-4017
1873-2550
DOI:10.1016/j.vetpar.2006.02.033