Effects of Temperature and Viscosity on R67 Dihydrofolate Reductase Catalysis

R67 dihydrofolate reductase (DHFR) is a novel homotetrameric protein that possesses 222 symmetry and a single, voluminous active site pore. This symmetry poses numerous limitations on catalysis; for example, two dihydrofolate (DHF) molecules or two NADPH molecules, or one substrate plus one cofactor...

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Bibliographic Details
Published in:Biochemistry (Easton) 2006-05, Vol.45 (21), p.6596-6605
Main Authors: Chopra, Shaileja, Lynch, Rachel, Kim, Su-Hwa, Jackson, Michael, Howell, Elizabeth E
Format: Article
Language:English
Subjects:
Catalysis
Fluorescence
Models, Molecular
Recombinant Proteins - chemistry
Recombinant Proteins - metabolism
Substrate Specificity
Temperature
Tetrahydrofolate Dehydrogenase - chemistry
Tetrahydrofolate Dehydrogenase - metabolism
Thermodynamics
Viscosity
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Summary:R67 dihydrofolate reductase (DHFR) is a novel homotetrameric protein that possesses 222 symmetry and a single, voluminous active site pore. This symmetry poses numerous limitations on catalysis; for example, two dihydrofolate (DHF) molecules or two NADPH molecules, or one substrate plus one cofactor can bind. Only the latter combination leads to catalysis. To garner additional information on how this enzyme facilitates transition-state formation, the temperature dependence of binding and catalysis was monitored. The binding of NADPH and DHF is enthalpy-driven. Previous primary isotope effect studies indicate hydride transfer is at least partially rate-determining. Accordingly, the activation energy associated with transition-state formation was measured and is found to be 6.9 kcal/mol (ΔH ⧧ 25 = 6.3 kcal/mol). A large entropic component is also found associated with catalysis, TΔS ⧧ 25 = −11.3 kcal/mol. The poor substrate, dihydropteroate, binds more weakly than dihydrofolate (ΔΔG = 1.4 kcal/mol) and displays a large loss in the binding enthalpy value (ΔΔH = 3.8 kcal/mol). The k cat value for dihydropteroate reduction is decreased 1600-fold compared to DHF usage. This effect appears to derive mostly from the ΔΔH difference in binding, demonstrating that the glutamate tail is important for catalysis. This result is surprising, as the para-aminobenzoyl-glutamate tail of DHF has been previously shown to be disordered by both NMR and crystallography studies. Viscosity studies were also performed and confirmed that the hydride transfer rate is not sensitive to sucrose addition. Surprisingly, binding of DHF, by both K m and K d determination, was found to be sensitive to added viscogens, suggesting a role for water in DHF binding.
ISSN:0006-2960
1520-4995
DOI:10.1021/bi052504l