Effects of aspirin plus alpha-tocopherol on brain slices damage after hypoxia-reoxygenation in rats with type 1-like diabetes mellitus

Diabetes mellitus is a risk factor for cerebrovascular ischemic disease. Aspirin (acetylsalicylic acid) is the most widely used drug for the secondary prevention of thrombotic phenomena. It has been also recently demonstrated that α-tocopherol influenced in vitro the antiplatelet effect of aspirin....

Full description

Saved in:
Bibliographic Details
Published in:Neuroscience letters 2006-06, Vol.400 (3), p.252-257
Main Authors: González-Correa, J.A., Arrebola, M.M., Cansino, A.L., Muñoz-Marín, J., Guerrero, A., Sánchez de la Cuesta, F., De La Cruz, J.P.
Format: Article
Language:English
Subjects:
Acetylsalicylic acid
Alpha-tocopherol
alpha-Tocopherol - administration & dosage
Animals
Antioxidants - administration & dosage
Aspirin - administration & dosage
Biological and medical sciences
Brain hypoxia
Brain Ischemia - complications
Brain Ischemia - diagnosis
Brain Ischemia - metabolism
Brain Ischemia - prevention & control
Cells, Cultured
Diabetes Mellitus, Type 1 - complications
Diabetes Mellitus, Type 1 - diagnosis
Diabetes Mellitus, Type 1 - drug therapy
Diabetes Mellitus, Type 1 - metabolism
Diabetes. Impaired glucose tolerance
Drug Combinations
Endocrine pancreas. Apud cells (diseases)
Endocrinopathies
Etiopathogenesis. Screening. Investigations. Target tissue resistance
Male
Medical sciences
Neuroprotective Agents - administration & dosage
Nitric oxide
Oxidative stress
Platelet Aggregation Inhibitors - administration & dosage
Rats
Rats, Wistar
Reperfusion Injury - complications
Reperfusion Injury - diagnosis
Reperfusion Injury - metabolism
Reperfusion Injury - prevention & control
Treatment Outcome
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Diabetes mellitus is a risk factor for cerebrovascular ischemic disease. Aspirin (acetylsalicylic acid) is the most widely used drug for the secondary prevention of thrombotic phenomena. It has been also recently demonstrated that α-tocopherol influenced in vitro the antiplatelet effect of aspirin. The aim of the present study is to evaluate the effects aspirin plus α-tocopherol on cerebral oxidative stress, prostaglandin production and the nitric oxide pathway in a model of hypoxia-reoxygenation in rat brain slices. Our results show an imbalance in brain oxidative status (reflected mainly as the increase in lipid peroxides) as a result of diabetes itself rather than a failure of the glutathione-based antioxidant system. Moreover, our results also show a higher concentration of prostaglandins in the brain of diabetic animals and a higher nitric oxide concentration, mainly through a high iNOS activity. After 180 min of post-hypoxia reoxygenation, LDH activity was 40.6% higher in animals with diabetes, in comparison to non-diabetic animals. The increase of the LDH efflux observed in non-treated rats was reduced by 31.2% with aspirin, by 34.7% with α-tocopherol and by 69.8% with the association aspirin-α-tocopherol. The accumulation of prostaglandin E 2 observed in diabetic non-treated rats was reduced statistically after the treatment with aspirin (34.2% inhibition), α-tocopherol (19.3% inhibition) or the association aspirin-α-tocopherol (54.4% inhibition). Nitric oxide production after 180 min reoxygenation was significantly reduced in aspirin (36.4%), α-tocopherol (22.7%) and aspirin-α-tocopherol (77.8%) treated rats with respect to diabetic non-treated animals; this was related mainly with a reduction in iNOS activity. The association between aspirin and alpha tocopherol could protects against brain ischemic-reperfusion damage with a better profile than aspirin alone.
ISSN:0304-3940
1872-7972
DOI:10.1016/j.neulet.2006.02.059