The extracorporeal perfusion of the swine uterus as an experimental model: The effect of tocolytic drugs

Comparison of the effect of tocolytic drugs on isolated swine uterus preparations. Forty swine uteri were perfused with the aim to preserve a viable organ, which should be responsive to oxytocic hormones and tocolytic pharmaca. An intrauterine catheter recorded the pressure changes. After initiation...

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Bibliographic Details
Published in:European journal of obstetrics & gynecology and reproductive biology 2006-05, Vol.126 (1), p.56-62
Main Authors: Maltaris, Theodoros, Dragonas, Charalampos, Hoffmann, Inge, Mueller, Andreas, Schild, Ralf L., Schmidt, Werner, Beckmann, Matthias W., Dittrich, Ralf
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Dinoprostone - pharmacology
Extracorporeal perfusion
Female
Fenoterol - pharmacology
Gynecology. Andrology. Obstetrics
Hormones
Medical sciences
Models, Animal
Muscle Contraction - drug effects
Muscle Contraction - physiology
Myometrium
Myometrium - drug effects
Myometrium - physiology
Organ Culture Techniques
Perfusion - methods
Swine
Swine uterus
Tissue Preservation - methods
Tocolysis
Tocolytic Agents - pharmacology
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Summary:Comparison of the effect of tocolytic drugs on isolated swine uterus preparations. Forty swine uteri were perfused with the aim to preserve a viable organ, which should be responsive to oxytocic hormones and tocolytic pharmaca. An intrauterine catheter recorded the pressure changes. After initiation of rhythmical uterine contractions we administered known tocolytic drugs (fenoterol, ritodrine, terbutaline, propofol, acetylsalicylic acid, alcohol, atosiban, verapamil, and glyceryl trinitrate) in various concentrations. Perfusate pH and lactate, partial oxygen and carbon dioxide tensions, and oxygen saturation in the perfusate showed good preservation of the organ for up to 8 h. All substances showed a tocolytic effect on the swine uterus. The effect varied substantially with regard to the length of the contraction free intervals, which was our main effect parameter. Fenoterol, acetylsalicylic acid, and alcohol showed the most and glyceryl trinitrate the least powerful effect. A direct comparison of various tocolytic substances in the same experimental model showed the best effect for fenoterol. Furthermore, we could demonstrate that the swine uterus perfusion system is a suitable model to study the influence of various conditions like the administration of drugs or the induction of oxidative stress on the uterus function.
ISSN:0301-2115
1872-7654
DOI:10.1016/j.ejogrb.2005.07.026