Apolipoprotein A‐I increases association of cytosolic cholesterol and caveolin‐1 with microtubule cytoskeletons in rat astrocytes

Apolipoprotein (apo) A‐I induces rapid translocation of protein kinase Cα and phospholipase Cγ, and slow translocation of caveolin‐1 and newly synthesized cholesterol to the cytosolic lipid‐protein particle (CLPP) fraction in rat astrocytes. In order to understand the function of CLPP, we investigat...

Full description

Saved in:
Bibliographic Details
Published in:Journal of neurochemistry 2006-05, Vol.97 (4), p.1034-1043
Main Authors: Ito, Jin‐ichi, Kheirollah, Alireza, Nagayasu, Yuko, Lu, Rui, Kato, Koichi, Yokoyama, Shinji
Format: Article
Language:English
Subjects:
Animals
Apolipoprotein A-I - metabolism
Apolipoprotein A-I - pharmacology
apolipoprotein A‐I
astrocytes
Astrocytes - drug effects
Astrocytes - metabolism
Astrocytes - ultrastructure
Biological and medical sciences
Caveolae - drug effects
Caveolae - metabolism
Caveolae - ultrastructure
Caveolin 1 - chemistry
Caveolin 1 - metabolism
caveolin‐1
Cells, Cultured
Cholesterol
Cholesterol - metabolism
Cytoskeleton
Cytoskeleton - drug effects
Cytoskeleton - metabolism
Cytoskeleton - ultrastructure
Cytosol - drug effects
Cytosol - metabolism
Cytosol - ultrastructure
cytosolic lipid‐protein particle
Degenerative and inherited degenerative diseases of the nervous system. Leukodystrophies. Prion diseases
Fundamental and applied biological sciences. Psychology
Isolated neuron and nerve. Neuroglia
Lipids
Lipoproteins, HDL - metabolism
Medical sciences
Microscopy, Electron, Transmission
microtubule
Microtubules - drug effects
Microtubules - metabolism
Microtubules - ultrastructure
Neurology
Paclitaxel - pharmacology
Peptides - metabolism
Peptides - pharmacology
Protein Kinase C-alpha - metabolism
Protein Structure, Tertiary - physiology
Protein Transport - drug effects
Protein Transport - physiology
Proteins
Rats
Rats, Wistar
Rodents
Subcellular Fractions - metabolism
Tubulin - metabolism
Up-Regulation - drug effects
Up-Regulation - physiology
Vertebrates: nervous system and sense organs
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Apolipoprotein (apo) A‐I induces rapid translocation of protein kinase Cα and phospholipase Cγ, and slow translocation of caveolin‐1 and newly synthesized cholesterol to the cytosolic lipid‐protein particle (CLPP) fraction in rat astrocytes. In order to understand the function of CLPP, we investigated the interaction with cytoskeletons of CLPP‐related proteins such as caveolin‐1 and protein kinase Cα and of CLPP‐related lipids in rat astrocytes. Under the conditions that microtubules were depolymerized, association of cytosolic caveolin‐1 with protein kinase Cα and α‐tubulin was enhanced when the cells were treated with apoA‐I for 5 min. This association was suppressed by a scaffolding domain‐peptide of caveolin‐1. Association with the microtubule‐like filaments of cytosolic lipids, caveolin‐1 and protein kinase Cα was also increased by the apoA‐I treatment and inhibited by the scaffolding domain peptide. Paclitaxel (taxol), a compound to stabilize microtubules, suppressed the apoA‐I‐mediated intracellular translocation and release from the cells of the de novo synthesized cholesterol and phospholipid. The findings suggested that the association of CLPP with microtubules is mediated by a scaffolding domain of caveolin‐1, induced by apoA‐I and involved in regulation of intracellular cholesterol trafficking for assembly of cellular lipids to apoA‐I–high‐density lipoprotein (HDL).
ISSN:0022-3042
1471-4159
DOI:10.1111/j.1471-4159.2006.03805.x