Identification of 18 new transcribed retrotransposons in Schistosoma mansoni

This work describes 18 new transcribed retrotransposons of the blood fluke Schistosoma mansoni. Among them, 9 were LTR, 8 non-LTR, and 1 Penelope-like element (PLE) retrotransposon. Sequences were generated by in silico reconstruction using S. mansoni ESTs and transcripts obtained by rapid amplifica...

Full description

Saved in:
Bibliographic Details
Published in:Biochemical and biophysical research communications 2005-07, Vol.333 (1), p.230-240
Main Authors: DeMarco, Ricardo, Machado, Abimael A., Bisson-Filho, Alexandre W., Verjovski-Almeida, Sergio
Format: Article
Language:English
Subjects:
Animals
Chromosome Mapping - methods
DNA Transposable Elements - genetics
Evolution, Molecular
Genetic Variation - genetics
LTR retrotransposon
Non-LTR retrotransposon
Phylogenetic analyses
R2 family retrotransposon
Schistosoma mansoni
Schistosoma mansoni - genetics
Sequence Analysis, DNA - methods
Transcribed retrotransposons
Trematoda
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:This work describes 18 new transcribed retrotransposons of the blood fluke Schistosoma mansoni. Among them, 9 were LTR, 8 non-LTR, and 1 Penelope-like element (PLE) retrotransposon. Sequences were generated by in silico reconstruction using S. mansoni ESTs and transcripts obtained by rapid amplification of cDNA ends, complemented in some cases by sequencing of genomic clones amplified by PCR. A novel element from the ancient R2/R4/CRE transposon group is described for the first time in S. mansoni. In addition, one non-LTR retrotransposon family displays long (40–450 bp) 3′-UTR with at least six different transcribed sequences among the copies, five LTR retrotransposons have abundantly transcribed incomplete copies lacking the sequence segment coding for the reverse transcriptase domain, and four non-LTR retrotransposons code for DNA-binding PHD domains that may give them a differential targeting. These results allow for a comprehensive description of the transcribed retrotransposon diversity of this complex human parasite.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2005.05.080