The effect of incorporating RGD adhesive peptide in polyethylene glycol diacrylate hydrogel on osteogenesis of bone marrow stromal cells

Advances in tissue engineering require biofunctional scaffolds that can not only provide cells with structural support, but also interact with cells in a biological manner. To achieve this goal, a frequently used cell adhesion peptide Arg–Gly–Asp (RGD) was covalently incorporated into poly(ethylene...

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Bibliographic Details
Published in:Biomaterials 2005-10, Vol.26 (30), p.5991-5998
Main Authors: Yang, Fan, Williams, Christopher G., Wang, Dong-an, Lee, Hyukjin, Manson, Paul N., Elisseeff, Jennifer
Format: Article
Language:English
Subjects:
Alkaline Phosphatase - chemistry
Alkaline Phosphatase - metabolism
Animals
Biocompatible Materials - chemistry
Bone Marrow - metabolism
Bone Marrow Cells - cytology
Cell Adhesion
DNA - chemistry
Goats
Hydrogel
Hydrogel, Polyethylene Glycol Dimethacrylate - chemistry
Hydrogels - chemistry
Marrow stromal cells
Models, Statistical
Oligopeptides - chemistry
Oligopeptides - pharmacology
Osteocalcin - chemistry
Osteocalcin - metabolism
Osteogenesis
Peptides - chemistry
Phosphorus - chemistry
Photopolymerizing
Polyethylene Glycols - chemistry
Reverse Transcriptase Polymerase Chain Reaction
RGD
RNA - chemistry
Stromal Cells - cytology
Time Factors
Tissue Engineering - methods
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Summary:Advances in tissue engineering require biofunctional scaffolds that can not only provide cells with structural support, but also interact with cells in a biological manner. To achieve this goal, a frequently used cell adhesion peptide Arg–Gly–Asp (RGD) was covalently incorporated into poly(ethylene glycol) diacrylate (PEODA) hydrogel and its dosage effect (0.025, 1.25 and 2.5 m m) on osteogenesis of marrow stromal cells in a three-dimensional environment was examined. Expression of bone-related markers, osteocalcin (OCN) and Alkaline phosphatase (ALP), increased significantly as the RGD concentration increased. Compared with no RGD, 2.5 m m RGD group showed a 1344% increase in ALP production and a 277% increase in OCN accumulation in the medium. RGD helped MSCs maintain cbfa-1 expression when shifted from a two-dimensional environment to a three-dimensional environment. Soluble RGD was found to completely block the mineralization of marrow stromal cells, as manifested by quantitative calcium assay, phosphorus elemental analysis and Von Kossa staining. In conclusion, we have demonstrated that RGD-conjugated PEODA hydrogel promotes the osteogenesis of MSCs in a dosage-dependent manner, with 2.5 m m being optimal concentration.
ISSN:0142-9612
1878-5905
DOI:10.1016/j.biomaterials.2005.03.018