Secreted antigens of Mycobacterium avium subspecies paratuberculosis as prominent immune targets

We here describe the identification and characterization of three novel secreted Mycobacterium avium subsp. paratuberculosis antigens of 9, 15 and 34 kDa (Map2609, Map2942c and Map0210c, respectively) by screening a genomic expression library with a serum of a naturally infected clinical cow. The 9,...

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Bibliographic Details
Published in:Veterinary microbiology 2006-05, Vol.114 (3), p.337-344
Main Authors: Willemsen, Peter T.J., Westerveen, José, Dinkla, Annemieke, Bakker, Douwe, van Zijderveld, Fred G., Thole, Jelle E.R.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
analytical kits
animal diseases
Animals
antibodies
Antibodies, Bacterial - blood
antibody detection
antigen detection
antigen-antibody reactions
Antigens
Antigens, Bacterial - chemistry
Antigens, Bacterial - immunology
bacterial antigens
Base Sequence
Biological and medical sciences
blood serum
Cattle
Cattle Diseases - immunology
Cattle Diseases - microbiology
cows
disease detection
disease diagnosis
Electrophoresis, Polyacrylamide Gel - veterinary
Enzyme-Linked Immunosorbent Assay - veterinary
Fundamental and applied biological sciences. Psychology
gene expression
genomics
Microbiology
Molecular Sequence Data
Molecular Weight
Mycobacterium avium
Mycobacterium avium subsp. paratuberculosis
Mycobacterium avium subsp. paratuberculosis - immunology
Mycobacterium avium subsp. paratuberculosis - metabolism
Mycobacterium tuberculosis
new methods
Paratuberculosis
Paratuberculosis - immunology
Paratuberculosis - microbiology
ruminants
screening
Secreted
secretion
veterinary medicine
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Summary:We here describe the identification and characterization of three novel secreted Mycobacterium avium subsp. paratuberculosis antigens of 9, 15 and 34 kDa (Map2609, Map2942c and Map0210c, respectively) by screening a genomic expression library with a serum of a naturally infected clinical cow. The 9, 15 and 34 kDa antigens display strong homology to previously described M. tuberculosis antigens, TB8.4, MPT53 and Erp, respectively. Furthermore, these antigens were shown to be recognized by antibodies from infected cattle, when tested with a limited number of sera from subclinical ( n = 7) and clinical ( n = 3) infected cattle.
ISSN:0378-1135
1873-2542
DOI:10.1016/j.vetmic.2005.12.005