Structural and functional characterization of noncoding repetitive RNAs transcribed in stressed human cells

Thermal and chemical stresses induce the formation in human cells of novel and transient nuclear structures called nuclear stress bodies (nSBs). These contain heat shock factor 1 (HSF-1) and a specific subset of pre-mRNA processing factors. Nuclear stress bodies are assembled on specific pericentrom...

Full description

Saved in:
Bibliographic Details
Published in:Molecular biology of the cell 2005-06, Vol.16 (6), p.2597-2604
Main Authors: Valgardsdottir, Rut, Chiodi, Ilaria, Giordano, Manuela, Cobianchi, Fabio, Riva, Silvano, Biamonti, Giuseppe
Format: Article
Language:English
Subjects:
Animals
Argonaute Proteins
Cell Fusion
Cell Nucleus - genetics
Cell Nucleus - metabolism
Cell Nucleus Structures - metabolism
Coculture Techniques
DNA, Satellite - chemistry
DNA, Satellite - metabolism
DNA-Binding Proteins
Dose-Response Relationship, Drug
Down-Regulation
Euchromatin - genetics
Euchromatin - metabolism
Eukaryotic Initiation Factor-2
Heat Shock Transcription Factors
Heat-Shock Response
HeLa Cells
Heterochromatin - genetics
Heterochromatin - metabolism
Humans
Mice
NIH 3T3 Cells
Oligonucleotides, Antisense - pharmacology
Peptide Initiation Factors - metabolism
RNA, Small Interfering - pharmacology
RNA, Untranslated - chemistry
RNA, Untranslated - genetics
RNA, Untranslated - metabolism
RNA-Induced Silencing Complex
Sequence Analysis, RNA
Stress, Physiological - genetics
Stress, Physiological - metabolism
Transcription Factors
Transcription, Genetic
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Thermal and chemical stresses induce the formation in human cells of novel and transient nuclear structures called nuclear stress bodies (nSBs). These contain heat shock factor 1 (HSF-1) and a specific subset of pre-mRNA processing factors. Nuclear stress bodies are assembled on specific pericentromeric heterochromatic domains containing satellite III (SatIII) DNA. In response to stress, these domains change their epigenetic status from heterochromatin to euchromatin and are transcribed in poly-adenylated RNAs that remain associated with nSBs. In this article, we describe the cloning, sequencing, and functional characterization of these transcripts. They are composed of SatIII repeats and originate from the transcription of multiple sites within the SatIII arrays. Interestingly, the level of SatIII RNAs can be down-regulated both by antisense oligonucleotides and small interfering RNAs (siRNA). Knockdown of SatIII RNA by siRNAs requires the activity of Argonaute 2, a component of the RNA-induced silencing complex. Down-regulation of satellite III RNAs significantly affects the recruitment of RNA processing factors to nSBs without altering the association of HSF-1 with these structures nor the presence of acetylated histones within nSBs. Thus, satellite III RNAs have a major role in the formation of nSBs.
ISSN:1059-1524
1939-4586
DOI:10.1091/mbc.e04-12-1078