Integrative Properties of Radial Oblique Dendrites in Hippocampal CA1 Pyramidal Neurons

Although radial oblique dendrites are a major synaptic input site in CA1 pyramidal neurons, little is known about their integrative properties. We have used multisite two-photon glutamate uncaging to deliver different spatiotemporal input patterns to single branches while simultaneously recording th...

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Bibliographic Details
Published in:Neuron (Cambridge, Mass.) Mass.), 2006-04, Vol.50 (2), p.291-307
Main Authors: Losonczy, Attila, Magee, Jeffrey C.
Format: Article
Language:English
Subjects:
Animals
Brain
Calcium Channels - metabolism
Dendrites - physiology
Excitatory Postsynaptic Potentials - physiology
Expected values
Glutamic Acid - metabolism
MOLNEURO
Neurons
Patch-Clamp Techniques
Population
Pyramidal Cells - physiology
Rats
Rats, Sprague-Dawley
Receptors, N-Methyl-D-Aspartate - metabolism
Synapses - physiology
Synaptic Transmission - physiology
SYSBIO
SYSNEURO
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Summary:Although radial oblique dendrites are a major synaptic input site in CA1 pyramidal neurons, little is known about their integrative properties. We have used multisite two-photon glutamate uncaging to deliver different spatiotemporal input patterns to single branches while simultaneously recording the uncaging-evoked excitatory postsynaptic potentials and local Ca 2+ signals. Asynchronous input patterns sum linearly in spite of the spatial clustering and produce Ca 2+ signals that are mediated by NMDA receptors (NMDARs). Appropriately timed and sized input patterns (∼20 inputs within ∼6 ms) produce a supralinear summation due to the initiation of a dendritic spike. The Ca 2+ signals associated with synchronous input were larger and mediated by influx through both NMDARs and voltage-gated Ca 2+ channels (VGCCs). The oblique spike is a fast Na + spike whose duration is shaped by the coincident activation of NMDAR, VGCCs, and transient K + currents. Our results suggest that individual branches can function as single integrative compartments.
ISSN:0896-6273
1097-4199
DOI:10.1016/j.neuron.2006.03.016