Aspartate transport and metabolism in the protozoan parasite Trypanosoma cruzi

Aspartate is one of the compounds that induce the differentiation process of the non-infective epimastigote stage to the infective trypomastigote stage of the protozoan parasite Trypanosoma cruzi. l-aspartate is transported by both epimastigote and trypomastigote cells at the same rate, about 3.4 pm...

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Bibliographic Details
Published in:FEMS microbiology letters 2005-06, Vol.247 (1), p.65-71
Main Authors: Canepa, Gaspar E., Bouvier, León A., Urias, Ursula, Miranda, Mariana R., Colli, Walter, Alves, Maria Júlia M., Pereira, Claudio A.
Format: Article
Language:English
Subjects:
Adenylosuccinate Synthase - genetics
Adenylosuccinate Synthase - metabolism
Amino acid transporter
Amino acids
Animals
Asparaginase - genetics
Asparaginase - metabolism
Aspartate
Aspartate Aminotransferases - genetics
Aspartate Aminotransferases - metabolism
Aspartate-Ammonia Ligase - genetics
Aspartate-Ammonia Ligase - metabolism
Aspartic Acid - metabolism
Biological and medical sciences
Biological Transport
Catalysis
Chagas disease
Computational Biology
Differentiation
Glutamate
Human protozoal diseases
Infectious diseases
Kinetics
Medical sciences
Metabolism
Metabolite transport
Microbiology
Molecular Sequence Data
Parasites
Parasitic diseases
Protozoa
Protozoal diseases
Transportation systems
Trypanosoma cruzi
Trypanosoma cruzi - enzymology
Trypanosoma cruzi - genetics
Trypanosoma cruzi - metabolism
Trypanosomiasis
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Summary:Aspartate is one of the compounds that induce the differentiation process of the non-infective epimastigote stage to the infective trypomastigote stage of the protozoan parasite Trypanosoma cruzi. l-aspartate is transported by both epimastigote and trypomastigote cells at the same rate, about 3.4 pmol min −1 per 10 7 cells. Aspartate transport is only competed by glutamate suggesting that this transport system is specific for anionic amino acids. Aspartate uptake rates increase along the parasite growth curve, by amino acids starvation or pH decrease. The metabolic fate of the transported aspartate was predicted in silico by identification of seven putative genes coding for enzymes involved in aspartate metabolism that could be related to the differentiation process.
ISSN:0378-1097
1574-6968
DOI:10.1016/j.femsle.2005.04.029