Physical and Chemical Adsorption of Mucor javanicus Lipase on SBA-15 Mesoporous Silica. Synthesis, Structural Characterization, and Activity Performance

In this work a sample of SBA-15 mesoporous silica was synthesized and characterized by TEM, XRD, and N2 adsorption. The sample had a high value of specific surface area (1007 m2 g-1) and total pore volume (2.1 cm3 g-1). The pore diameter was 67 Å, so it was large enough to accommodate protein molecu...

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Bibliographic Details
Published in:Langmuir 2005-06, Vol.21 (12), p.5511-5516
Main Authors: Salis, Andrea, Meloni, Daniela, Ligas, Stefania, Casula, Maria F, Monduzzi, Maura, Solinas, Vincenzo, Dumitriu, Emil
Format: Article
Language:English
Subjects:
Adsorption
Chemistry
Colloidal state and disperse state
Enzymes, Immobilized - chemistry
Exact sciences and technology
General and physical chemistry
Hydrogen-Ion Concentration
Lipase - chemistry
Lipase - metabolism
Models, Chemical
Mucor - enzymology
Porosity
Porous materials
Silicon Dioxide - chemistry
Surface physical chemistry
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Summary:In this work a sample of SBA-15 mesoporous silica was synthesized and characterized by TEM, XRD, and N2 adsorption. The sample had a high value of specific surface area (1007 m2 g-1) and total pore volume (2.1 cm3 g-1). The pore diameter was 67 Å, so it was large enough to accommodate protein molecules inside the channels. Immobilization by physical adsorption of a commercial lipase preparation from Mucor javanicus was performed at different pH values (pH 5−8). pH 6 gave the highest lipase loading and hydrolytic activity of the corresponding biocatalyst. Chemical modification of the SBA-15 via glutardialdehyde allowed also the enzyme immobilization through chemical adsorption. This preparation was active toward tributyrin hydrolysis. On the contrary, very low activity toward triolein hydrolysis was observed. The reduction of the size of the channels due the immobilization process has been suggested as a possible explanation.
ISSN:0743-7463
1520-5827
DOI:10.1021/la047225y