Opsonic Requirements for the Respiratory Burst of Neutrophils against Giardia lamblia Trophozoites

Giardia lamblia is an important cause of parasitic diarrheal disease worldwide. Occasionally, polymorphonuclear neutrophils (PMNs) may participate as effector cells against Giardia lamblia. The present study was performed in order to examine the role of specific antibody and complement components in...

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Bibliographic Details
Published in:Archives of medical research 2006-05, Vol.37 (4), p.465-473
Main Authors: Arbo, Antonio, Pavía-Ruz, Noris, Santos, José Ignacio
Format: Article
Language:English
Subjects:
Animals
Antibodies, Monoclonal - immunology
Cell Adhesion
Giardia lamblia
Giardia lamblia - growth & development
Giardia lamblia - immunology
Humans
Neutrophils - cytology
Neutrophils - immunology
Neutrophils - metabolism
Opsonin Proteins - immunology
Opsonization
Polymorphonuclear neutrophil
Receptors, Immunologic - immunology
Respiratory Burst
Superoxide Dismutase - metabolism
Superoxides - metabolism
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Summary:Giardia lamblia is an important cause of parasitic diarrheal disease worldwide. Occasionally, polymorphonuclear neutrophils (PMNs) may participate as effector cells against Giardia lamblia. The present study was performed in order to examine the role of specific antibody and complement components in promoting the respiratory burst (RB) of PMNs against Giardia lamblia. PMNs from human adult volunteers were incubated with Giardia trophozoites in the presence of non-immune (NS) or hyperimmune (HS) serum (anti- Giardia titer, >1:1024). Adherence was scored visually on coverslide after staining with Giemsa. The ability of Giardia to trigger the oxidative response of PMNs was measured by the anion superoxide (O 2 −) production using a cytochrome C reduction method and by the luminol amplified chemiluminescence (CL) assay. Incubation with NS or HS increased Giardia adherence to PMNs from 6.9 ± 3.2% (basal adherence of Giardia incubated in buffer) to 39 ± 18.6% ( p
ISSN:0188-4409
1873-5487
DOI:10.1016/j.arcmed.2005.11.002