The replicative polymerases PolC and DnaE are required for theta replication of the Bacillus subtilis plasmid pBS72

1 Belarussian State University, Biological Faculty, Department of Genetics and Biotechnology, Minsk 220050, 4 Scorina Avenue, Belarus 2 Memorial Sloan-Kettering Cancer Center, NY 10021, USA 3 Laboratoire de Génétique Microbienne, Bâtiment des Biotechnologies, INRA, 78352 Jouy en Josas, France Corres...

Full description

Saved in:
Bibliographic Details
Published in:Microbiology (Society for General Microbiology) 2006-05, Vol.152 (5), p.1471-1478
Main Authors: Titok, Marina, Suski, Catherine, Dalmais, Berengere, Ehrlich, S. Dusko, Janniere, Laurent
Format: Article
Language:English
Subjects:
Bacillus subtilis
Bacillus subtilis - genetics
Bacterial Proteins - genetics
Bacterial Proteins - physiology
Bacteriology
Base Sequence
Biological and medical sciences
DNA Helicases - genetics
DNA Helicases - physiology
DNA Polymerase III - genetics
DNA Polymerase III - physiology
DNA Replication
DNA-Binding Proteins - genetics
DNA-Binding Proteins - physiology
DNA-Directed DNA Polymerase - genetics
DNA-Directed DNA Polymerase - physiology
Fundamental and applied biological sciences. Psychology
Gene Order
Genes, Bacterial
Metabolism. Enzymes
Microbiology
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
Plasmids - genetics
Replication
Replication Origin - genetics
Replication Origin - physiology
Trans-Activators - genetics
Trans-Activators - physiology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:1 Belarussian State University, Biological Faculty, Department of Genetics and Biotechnology, Minsk 220050, 4 Scorina Avenue, Belarus 2 Memorial Sloan-Kettering Cancer Center, NY 10021, USA 3 Laboratoire de Génétique Microbienne, Bâtiment des Biotechnologies, INRA, 78352 Jouy en Josas, France Correspondence Laurent Janniere laurent.janniere{at}jouy.inra.fr Plasmids are the tools of choice for studying bacterial functions involved in DNA maintenance. Here a genetic study on the replication of a novel, low-copy-number, Bacillus subtilis plasmid, pBS72, is reported. The results show that two plasmid elements, the initiator protein RepA and an iteron-containing origin, and at least nine host-encoded replication proteins, the primosomal proteins DnaB, DnaC, DnaD, DnaG and DnaI, the DNA polymerases DnaE and PolC, and the polymerase cofactors DnaN and DnaX, are required for pBS72 replication. On the contrary, the cellular initiators DnaA and PriA, the helicase PcrA and DNA polymerase I are dispensable. From this, it is inferred that pBS72 replication is of the theta type and is initiated by an original mechanism. Indirect evidence suggests that during this process the DnaC helicase might be delivered to the plasmid origin by the weakly active DnaD pathway stimulated by a predicted interaction between DnaC and a domain of RepA homologous to the major DnaC-binding domain of the cellular initiator DnaA. The plasmid pBS72 replication fork appears to require the same functions as the bacterial chromosome and the unrelated plasmid pAM 1. Most importantly, this replication machinery contains the two type C polymerases, PolC and DnaE. As the mechanism of initiation of the three genomes is substantially different, this suggests that both type C polymerases might be required in any Cairns replication in B. subtilis and presumably in other bacteria encoding PolC and DnaE. Abbreviations: CN, copy number On the CNRS staff.
ISSN:1350-0872
1465-2080
DOI:10.1099/mic.0.28693-0