Bone marrow stromal cells and resorbable collagen guidance tubes enhance sciatic nerve regeneration in mice

We evaluated peripheral nerve regeneration using a tubular nerve guide of resorbable collagen filled with either bone marrow-derived cells (BMDCs) in Dulbecco's cell culture medium (DMEM) or with DMEM alone (control). The control group received just the culture medium (vehicle). The left sciati...

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Bibliographic Details
Published in:Experimental neurology 2006-04, Vol.198 (2), p.457-468
Main Authors: Pereira Lopes, Fátima Rosalina, Camargo de Moura Campos, Lenira, Dias Corrêa, José, Balduino, Alex, Lora, Silvano, Langone, Francesco, Borojevic, Radovan, Blanco Martinez, Ana Maria
Format: Article
Language:English
Subjects:
Animals
Biocompatible Materials - therapeutic use
Biological and medical sciences
Blotting, Northern - methods
Bone Marrow Cells - physiology
Bone Marrow Cells - ultrastructure
Bone marrow-derived cells
Cells, Cultured
Collagen - pharmacology
Collagen - ultrastructure
Collagen tube
Cranial nerves. Peripheral nerves. Autonomic nervous system
Disease Models, Animal
Electron microscopy
Female
Gene Expression - physiology
Injuries of the nervous system and the skull. Diseases due to physical agents
Medical sciences
Mice
Mice, Inbred BALB C
Microscopy, Electron - methods
Motor Activity - physiology
Nerve Fibers, Myelinated - metabolism
Nerve Fibers, Myelinated - ultrastructure
Nerve Regeneration - drug effects
Nerve Regeneration - physiology
Neurosurgery
Peripheral nerve regeneration
Recovery of Function - physiology
Reverse Transcriptase Polymerase Chain Reaction - methods
RNA, Messenger - genetics
RNA, Messenger - metabolism
Schwann cells
Sciatic Nerve - cytology
Sciatic Nerve - drug effects
Sciatic Nerve - ultrastructure
Sciatic Neuropathy - pathology
Sciatic Neuropathy - surgery
Stromal Cells - physiology
Stromal Cells - ultrastructure
Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases
Time Factors
Transplantation
Traumas. Diseases due to physical agents
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Description
Summary:We evaluated peripheral nerve regeneration using a tubular nerve guide of resorbable collagen filled with either bone marrow-derived cells (BMDCs) in Dulbecco's cell culture medium (DMEM) or with DMEM alone (control). The control group received just the culture medium (vehicle). The left sciatic nerves of ten isogenic mice were transected and the tubular nerve guides were sutured to the end of the proximal and distal nerve stumps. Motor function was tested at 2, 4 and 6 weeks after surgery using the walking track test. The pawprints were analyzed and the print lengths (PL) were measured to evaluate functional recovery. After 6 weeks, mice were anesthetized, perfused transcardially with fixative containing aldehydes, and the sciatic nerves and tubes were dissected and processed for scanning and transmission electron microscopy. Scanning electron microscopy of the collagen tube revealed that the tube wall became progressively thinner after surgery, proving that the tube can be resorbed in vivo. Quantitative analysis of the regenerating nerves showed that the number of myelinated fibers and the myelin area were significantly increased in the experimental group. Also, motor function recovery was faster in animals that received the cell grafts. These results indicate that the collagen tube filled with BMDCs provided an adequate and favorable environment for the growth and myelination of regenerating axons compared to the collagen tube alone.
ISSN:0014-4886
1090-2430
DOI:10.1016/j.expneurol.2005.12.019