MIB-1 and MCM-2 immunohistochemical analysis does not aid in identification of serrated colorectal polyps with abnormal proliferation

We investigated the staining characteristics of serrated polyps with abnormal proliferation (SPAP) using MIB-1 and MCM-2 to determine if they could provide assistance in delineating SPAPs from traditional hyperplastic polyps (HPs). Using published morphologic criteria we reviewed H&E slides of 1...

Full description

Saved in:
Bibliographic Details
Published in:American journal of clinical pathology 2006-03, Vol.125 (3), p.407-412
Main Authors: GRAY, David, OBERMANN, Ellen C, EVANS, Mark, HARTMANN, Arndt, COOPER, Kumarasen, BLASZYK, Hagen
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biomarkers, Tumor - analysis
Cell Cycle Proteins - analysis
Cell Proliferation
Colonic Polyps - chemistry
Colonic Polyps - classification
Colonic Polyps - pathology
Colorectal Neoplasms - chemistry
Colorectal Neoplasms - classification
Colorectal Neoplasms - pathology
Diagnosis, Differential
Female
Gastroenterology. Liver. Pancreas. Abdomen
Humans
Hyperplasia - metabolism
Hyperplasia - pathology
Immunoenzyme Techniques
Intestinal Mucosa - chemistry
Intestinal Mucosa - pathology
Investigative techniques, diagnostic techniques (general aspects)
Ki-67 Antigen - analysis
Male
Medical sciences
Middle Aged
Minichromosome Maintenance Complex Component 2
Nuclear Proteins - analysis
Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques
Stomach. Duodenum. Small intestine. Colon. Rectum. Anus
Tumors
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We investigated the staining characteristics of serrated polyps with abnormal proliferation (SPAP) using MIB-1 and MCM-2 to determine if they could provide assistance in delineating SPAPs from traditional hyperplastic polyps (HPs). Using published morphologic criteria we reviewed H&E slides of 107 polyps from 80 patients. Thirty-nine (36.4%) polyps met the criteria for SPAP Within a given region, polyps in the transverse colon had the largest percentage of SPAPs (50.0%) followed by the right colon (40.9%). The majority of SPAPs (82.1%) and HPs (72.1%) showed MIB-1 staining confined to the basal third of the crypts. The majority of SPAPs (59.0%) and HPs (52.9%) showed MCM-2 staining extending into the apical third of the crypts. We do not recommend MIB-1 or MCM-2 staining to differentiate SPAPs from conventional HPs, since staining characteristics are not significantly different between the 2 groups, and frequent variable crypt staining within a given polyp is difficult to interpret.
ISSN:0002-9173
1943-7722
DOI:10.1309/E1BG63G8XAW32DNE