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Arabidopsis Hsa32, a Novel Heat Shock Protein, Is Essential for Acquired Thermotolerance during Long Recovery after Acclimation
Plants and animals share similar mechanisms in the heat shock (HS) response, such as synthesis of the conserved HS proteins (Hsps). However, because plants are confined to a growing environment, in general they require unique features to cope with heat stress. Here, we report on the analysis of the...
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| Published in: | Plant physiology (Bethesda) 2006-04, Vol.140 (4), p.1297-1305 |
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| container_end_page | 1305 |
| container_issue | 4 |
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| container_title | Plant physiology (Bethesda) |
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| creator | Charng, Yee-yung Liu, Hsiang-chin Liu, Nai-yu Hsu, Fu-chiun Ko, Swee-suak |
| description | Plants and animals share similar mechanisms in the heat shock (HS) response, such as synthesis of the conserved HS proteins (Hsps). However, because plants are confined to a growing environment, in general they require unique features to cope with heat stress. Here, we report on the analysis of the function of a novel Hsp, heat-stress-associated 32-kD protein (Hsa32), which is highly conserved in land plants but absent in most other organisms. The gene responds to HS at the transcriptional level in moss (Physcomitrella patens), Arabidopsis (Arabidopsis thaliana), and rice (Oryza sativa). Like other Hsps, Hsa32 protein accumulates greatly in Arabidopsis seedlings after HS treatment. Disruption of Hsa32 by T-DNA insertion does not affect growth and development under normal conditions. However, the acquired thermotolerance in the knockout line was compromised following a long recovery period (>24 h) after acclimation HS treatment, when a severe HS challenge killed the mutant but not the wild-type plants, but no significant difference was observed if they were challenged within a short recovery period. Quantitative hypocotyl elongation assay also revealed that thermotolerance decayed faster in the absence of Hsa32 after a long recovery. Similar results were obtained in Arabidopsis transgenic plants with Hsa32 expression suppressed by RNA interference. Microarray analysis of the knockout mutant indicates that only the expression of Hsa32 was significantly altered in HS response. Taken together, our results suggest that Hsa32 is required not for induction but rather maintenance of acquired thermotolerance, a feature that could be important to plants. |
| doi_str_mv | 10.1104/pp.105.074898 |
| format | article |
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However, because plants are confined to a growing environment, in general they require unique features to cope with heat stress. Here, we report on the analysis of the function of a novel Hsp, heat-stress-associated 32-kD protein (Hsa32), which is highly conserved in land plants but absent in most other organisms. The gene responds to HS at the transcriptional level in moss (Physcomitrella patens), Arabidopsis (Arabidopsis thaliana), and rice (Oryza sativa). Like other Hsps, Hsa32 protein accumulates greatly in Arabidopsis seedlings after HS treatment. Disruption of Hsa32 by T-DNA insertion does not affect growth and development under normal conditions. However, the acquired thermotolerance in the knockout line was compromised following a long recovery period (>24 h) after acclimation HS treatment, when a severe HS challenge killed the mutant but not the wild-type plants, but no significant difference was observed if they were challenged within a short recovery period. Quantitative hypocotyl elongation assay also revealed that thermotolerance decayed faster in the absence of Hsa32 after a long recovery. Similar results were obtained in Arabidopsis transgenic plants with Hsa32 expression suppressed by RNA interference. Microarray analysis of the knockout mutant indicates that only the expression of Hsa32 was significantly altered in HS response. Taken together, our results suggest that Hsa32 is required not for induction but rather maintenance of acquired thermotolerance, a feature that could be important to plants.</description><identifier>ISSN: 1532-2548</identifier><identifier>ISSN: 0032-0889</identifier><identifier>EISSN: 1532-2548</identifier><identifier>DOI: 10.1104/pp.105.074898</identifier><identifier>PMID: 16500991</identifier><identifier>CODEN: PPHYA5</identifier><language>eng</language><publisher>Rockville, MD: American Society of Plant Biologists</publisher><subject>acclimation ; Acclimatization ; Antibodies ; Arabidopsis - anatomy & histology ; Arabidopsis - genetics ; Arabidopsis - metabolism ; Arabidopsis Proteins - genetics ; Arabidopsis Proteins - metabolism ; Arabidopsis Proteins - physiology ; Arabidopsis thaliana ; Base Sequence ; Biological and medical sciences ; Conserved Sequence ; DNA, Bacterial - genetics ; Environmental Stress and Adaptation to Stress ; Fundamental and applied biological sciences. Psychology ; gene expression regulation ; Gene Expression Regulation, Plant ; Genes ; Genes. Genome ; Heat shock proteins ; Heat tolerance ; heat treatment ; Heat-Shock Proteins - genetics ; Heat-Shock Proteins - metabolism ; Heat-Shock Proteins - physiology ; Heat-Shock Response ; heat-stress-associated protein ; Hypocotyls ; Molecular and cellular biology ; Molecular genetics ; Molecular Sequence Data ; Phenotypes ; plant proteins ; Plants ; Rice ; RNA, Messenger - metabolism ; Seedlings ; Time Factors ; transcription (genetics)</subject><ispartof>Plant physiology (Bethesda), 2006-04, Vol.140 (4), p.1297-1305</ispartof><rights>Copyright 2006 American Society of Plant Biologists</rights><rights>2006 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c509t-916d3328989903c7dc06257d1c2c615a7ccc270a0a25658727e0251cef482f3</citedby><cites>FETCH-LOGICAL-c509t-916d3328989903c7dc06257d1c2c615a7ccc270a0a25658727e0251cef482f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/20205693$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/20205693$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>315,786,790,27957,27958,58593,58826</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17700331$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16500991$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Charng, Yee-yung</creatorcontrib><creatorcontrib>Liu, Hsiang-chin</creatorcontrib><creatorcontrib>Liu, Nai-yu</creatorcontrib><creatorcontrib>Hsu, Fu-chiun</creatorcontrib><creatorcontrib>Ko, Swee-suak</creatorcontrib><title>Arabidopsis Hsa32, a Novel Heat Shock Protein, Is Essential for Acquired Thermotolerance during Long Recovery after Acclimation</title><title>Plant physiology (Bethesda)</title><addtitle>Plant Physiol</addtitle><description>Plants and animals share similar mechanisms in the heat shock (HS) response, such as synthesis of the conserved HS proteins (Hsps). However, because plants are confined to a growing environment, in general they require unique features to cope with heat stress. Here, we report on the analysis of the function of a novel Hsp, heat-stress-associated 32-kD protein (Hsa32), which is highly conserved in land plants but absent in most other organisms. The gene responds to HS at the transcriptional level in moss (Physcomitrella patens), Arabidopsis (Arabidopsis thaliana), and rice (Oryza sativa). Like other Hsps, Hsa32 protein accumulates greatly in Arabidopsis seedlings after HS treatment. Disruption of Hsa32 by T-DNA insertion does not affect growth and development under normal conditions. However, the acquired thermotolerance in the knockout line was compromised following a long recovery period (>24 h) after acclimation HS treatment, when a severe HS challenge killed the mutant but not the wild-type plants, but no significant difference was observed if they were challenged within a short recovery period. Quantitative hypocotyl elongation assay also revealed that thermotolerance decayed faster in the absence of Hsa32 after a long recovery. Similar results were obtained in Arabidopsis transgenic plants with Hsa32 expression suppressed by RNA interference. Microarray analysis of the knockout mutant indicates that only the expression of Hsa32 was significantly altered in HS response. Taken together, our results suggest that Hsa32 is required not for induction but rather maintenance of acquired thermotolerance, a feature that could be important to plants.</description><subject>acclimation</subject><subject>Acclimatization</subject><subject>Antibodies</subject><subject>Arabidopsis - anatomy & histology</subject><subject>Arabidopsis - genetics</subject><subject>Arabidopsis - metabolism</subject><subject>Arabidopsis Proteins - genetics</subject><subject>Arabidopsis Proteins - metabolism</subject><subject>Arabidopsis Proteins - physiology</subject><subject>Arabidopsis thaliana</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Conserved Sequence</subject><subject>DNA, Bacterial - genetics</subject><subject>Environmental Stress and Adaptation to Stress</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>gene expression regulation</subject><subject>Gene Expression Regulation, Plant</subject><subject>Genes</subject><subject>Genes. Genome</subject><subject>Heat shock proteins</subject><subject>Heat tolerance</subject><subject>heat treatment</subject><subject>Heat-Shock Proteins - genetics</subject><subject>Heat-Shock Proteins - metabolism</subject><subject>Heat-Shock Proteins - physiology</subject><subject>Heat-Shock Response</subject><subject>heat-stress-associated protein</subject><subject>Hypocotyls</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>Phenotypes</subject><subject>plant proteins</subject><subject>Plants</subject><subject>Rice</subject><subject>RNA, Messenger - metabolism</subject><subject>Seedlings</subject><subject>Time Factors</subject><subject>transcription (genetics)</subject><issn>1532-2548</issn><issn>0032-0889</issn><issn>1532-2548</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><recordid>eNpNkUFP3DAQhaOqVaG0xx7b-lJOZBnbsR0fV4h2kVa0Yuk5Ms4ETLNxsB0kTvz1epUV9GI_6X1-mnkuis8UFpRCdTqOCwpiAaqqdf2mOKSCs5KJqn77nz4oPsR4DwCU0-p9cUClANCaHhbPy2BuXOvH6CJZRcPZCTHk0j9iT1ZoEtncefuX_A4-oRtOyEUk5zHikJzpSecDWdqHyQVsyfUdhq1PvsdgBouknYIbbsna5-MKbU4MT8R0CXdvbO-2Jjk_fCzedaaP-Gl_HxWbH-fXZ6ty_evnxdlyXVoBOpWaypZzllfUGrhVrQXJhGqpZVZSYZS1likwYJiQolZMITBBLXZVzTp-VBzPqWPwDxPG1GxdtNj3ZkA_xUaqWgLVLIPlDNrgYwzYNWPIk4anhkKz67sZxyxFM_ed-a_74Olmi-0rvS84A9_3gInW9N2uGxdfOaUAON9xX2buPiYfXnwGDITUPPvfZr8zvjG3IWf82bD8n0BBapAV_wc-2ppH</recordid><startdate>20060401</startdate><enddate>20060401</enddate><creator>Charng, Yee-yung</creator><creator>Liu, Hsiang-chin</creator><creator>Liu, Nai-yu</creator><creator>Hsu, Fu-chiun</creator><creator>Ko, Swee-suak</creator><general>American Society of Plant Biologists</general><general>American Society of Plant Physiologists</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20060401</creationdate><title>Arabidopsis Hsa32, a Novel Heat Shock Protein, Is Essential for Acquired Thermotolerance during Long Recovery after Acclimation</title><author>Charng, Yee-yung ; Liu, Hsiang-chin ; Liu, Nai-yu ; Hsu, Fu-chiun ; Ko, Swee-suak</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c509t-916d3328989903c7dc06257d1c2c615a7ccc270a0a25658727e0251cef482f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>acclimation</topic><topic>Acclimatization</topic><topic>Antibodies</topic><topic>Arabidopsis - anatomy & histology</topic><topic>Arabidopsis - genetics</topic><topic>Arabidopsis - metabolism</topic><topic>Arabidopsis Proteins - genetics</topic><topic>Arabidopsis Proteins - metabolism</topic><topic>Arabidopsis Proteins - physiology</topic><topic>Arabidopsis thaliana</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Conserved Sequence</topic><topic>DNA, Bacterial - genetics</topic><topic>Environmental Stress and Adaptation to Stress</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>gene expression regulation</topic><topic>Gene Expression Regulation, Plant</topic><topic>Genes</topic><topic>Genes. Genome</topic><topic>Heat shock proteins</topic><topic>Heat tolerance</topic><topic>heat treatment</topic><topic>Heat-Shock Proteins - genetics</topic><topic>Heat-Shock Proteins - metabolism</topic><topic>Heat-Shock Proteins - physiology</topic><topic>Heat-Shock Response</topic><topic>heat-stress-associated protein</topic><topic>Hypocotyls</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>Phenotypes</topic><topic>plant proteins</topic><topic>Plants</topic><topic>Rice</topic><topic>RNA, Messenger - metabolism</topic><topic>Seedlings</topic><topic>Time Factors</topic><topic>transcription (genetics)</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Charng, Yee-yung</creatorcontrib><creatorcontrib>Liu, Hsiang-chin</creatorcontrib><creatorcontrib>Liu, Nai-yu</creatorcontrib><creatorcontrib>Hsu, Fu-chiun</creatorcontrib><creatorcontrib>Ko, Swee-suak</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Plant physiology (Bethesda)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Charng, Yee-yung</au><au>Liu, Hsiang-chin</au><au>Liu, Nai-yu</au><au>Hsu, Fu-chiun</au><au>Ko, Swee-suak</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Arabidopsis Hsa32, a Novel Heat Shock Protein, Is Essential for Acquired Thermotolerance during Long Recovery after Acclimation</atitle><jtitle>Plant physiology (Bethesda)</jtitle><addtitle>Plant Physiol</addtitle><date>2006-04-01</date><risdate>2006</risdate><volume>140</volume><issue>4</issue><spage>1297</spage><epage>1305</epage><pages>1297-1305</pages><issn>1532-2548</issn><issn>0032-0889</issn><eissn>1532-2548</eissn><coden>PPHYA5</coden><notes>ObjectType-Article-1</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-2</notes><notes>content type line 23</notes><abstract>Plants and animals share similar mechanisms in the heat shock (HS) response, such as synthesis of the conserved HS proteins (Hsps). However, because plants are confined to a growing environment, in general they require unique features to cope with heat stress. Here, we report on the analysis of the function of a novel Hsp, heat-stress-associated 32-kD protein (Hsa32), which is highly conserved in land plants but absent in most other organisms. The gene responds to HS at the transcriptional level in moss (Physcomitrella patens), Arabidopsis (Arabidopsis thaliana), and rice (Oryza sativa). Like other Hsps, Hsa32 protein accumulates greatly in Arabidopsis seedlings after HS treatment. Disruption of Hsa32 by T-DNA insertion does not affect growth and development under normal conditions. However, the acquired thermotolerance in the knockout line was compromised following a long recovery period (>24 h) after acclimation HS treatment, when a severe HS challenge killed the mutant but not the wild-type plants, but no significant difference was observed if they were challenged within a short recovery period. Quantitative hypocotyl elongation assay also revealed that thermotolerance decayed faster in the absence of Hsa32 after a long recovery. Similar results were obtained in Arabidopsis transgenic plants with Hsa32 expression suppressed by RNA interference. Microarray analysis of the knockout mutant indicates that only the expression of Hsa32 was significantly altered in HS response. Taken together, our results suggest that Hsa32 is required not for induction but rather maintenance of acquired thermotolerance, a feature that could be important to plants.</abstract><cop>Rockville, MD</cop><pub>American Society of Plant Biologists</pub><pmid>16500991</pmid><doi>10.1104/pp.105.074898</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | acclimation Acclimatization Antibodies Arabidopsis - anatomy & histology Arabidopsis - genetics Arabidopsis - metabolism Arabidopsis Proteins - genetics Arabidopsis Proteins - metabolism Arabidopsis Proteins - physiology Arabidopsis thaliana Base Sequence Biological and medical sciences Conserved Sequence DNA, Bacterial - genetics Environmental Stress and Adaptation to Stress Fundamental and applied biological sciences. Psychology gene expression regulation Gene Expression Regulation, Plant Genes Genes. Genome Heat shock proteins Heat tolerance heat treatment Heat-Shock Proteins - genetics Heat-Shock Proteins - metabolism Heat-Shock Proteins - physiology Heat-Shock Response heat-stress-associated protein Hypocotyls Molecular and cellular biology Molecular genetics Molecular Sequence Data Phenotypes plant proteins Plants Rice RNA, Messenger - metabolism Seedlings Time Factors transcription (genetics) |
| title | Arabidopsis Hsa32, a Novel Heat Shock Protein, Is Essential for Acquired Thermotolerance during Long Recovery after Acclimation |
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