Arabidopsis Hsa32, a Novel Heat Shock Protein, Is Essential for Acquired Thermotolerance during Long Recovery after Acclimation

Plants and animals share similar mechanisms in the heat shock (HS) response, such as synthesis of the conserved HS proteins (Hsps). However, because plants are confined to a growing environment, in general they require unique features to cope with heat stress. Here, we report on the analysis of the...

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Published in:Plant physiology (Bethesda) 2006-04, Vol.140 (4), p.1297-1305
Main Authors: Charng, Yee-yung, Liu, Hsiang-chin, Liu, Nai-yu, Hsu, Fu-chiun, Ko, Swee-suak
Format: Article
Language:English
Subjects:
acclimation
Acclimatization
Antibodies
Arabidopsis - anatomy & histology
Arabidopsis - genetics
Arabidopsis - metabolism
Arabidopsis Proteins - genetics
Arabidopsis Proteins - metabolism
Arabidopsis Proteins - physiology
Arabidopsis thaliana
Base Sequence
Biological and medical sciences
Conserved Sequence
DNA, Bacterial - genetics
Environmental Stress and Adaptation to Stress
Fundamental and applied biological sciences. Psychology
gene expression regulation
Gene Expression Regulation, Plant
Genes
Genes. Genome
Heat shock proteins
Heat tolerance
heat treatment
Heat-Shock Proteins - genetics
Heat-Shock Proteins - metabolism
Heat-Shock Proteins - physiology
Heat-Shock Response
heat-stress-associated protein
Hypocotyls
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
Phenotypes
plant proteins
Plants
Rice
RNA, Messenger - metabolism
Seedlings
Time Factors
transcription (genetics)
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Summary:Plants and animals share similar mechanisms in the heat shock (HS) response, such as synthesis of the conserved HS proteins (Hsps). However, because plants are confined to a growing environment, in general they require unique features to cope with heat stress. Here, we report on the analysis of the function of a novel Hsp, heat-stress-associated 32-kD protein (Hsa32), which is highly conserved in land plants but absent in most other organisms. The gene responds to HS at the transcriptional level in moss (Physcomitrella patens), Arabidopsis (Arabidopsis thaliana), and rice (Oryza sativa). Like other Hsps, Hsa32 protein accumulates greatly in Arabidopsis seedlings after HS treatment. Disruption of Hsa32 by T-DNA insertion does not affect growth and development under normal conditions. However, the acquired thermotolerance in the knockout line was compromised following a long recovery period (>24 h) after acclimation HS treatment, when a severe HS challenge killed the mutant but not the wild-type plants, but no significant difference was observed if they were challenged within a short recovery period. Quantitative hypocotyl elongation assay also revealed that thermotolerance decayed faster in the absence of Hsa32 after a long recovery. Similar results were obtained in Arabidopsis transgenic plants with Hsa32 expression suppressed by RNA interference. Microarray analysis of the knockout mutant indicates that only the expression of Hsa32 was significantly altered in HS response. Taken together, our results suggest that Hsa32 is required not for induction but rather maintenance of acquired thermotolerance, a feature that could be important to plants.
ISSN:1532-2548
0032-0889
1532-2548
DOI:10.1104/pp.105.074898