Vitamin E protects the insulin sensitivity and redox balance in rat L6 muscle cells exposed to oxidative stress

The effect of reactive oxygen species (ROS) on insulin action is unclear. This study was carried out to explore the effect of prolonged low grade oxidative stress and vitamin E treatment on cultured rat L6 muscle cells. L6 myotubes were incubated with vitamin E for 18 h and treated with H 2O 2 gener...

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Bibliographic Details
Published in:Clinica chimica acta 2006-05, Vol.367 (1), p.132-136
Main Authors: Vinayaga Moorthi, R., Bobby, Zachariah, Selvaraj, N., Sridhar, M.G.
Format: Article
Language:English
Subjects:
Animals
Biological Transport
Cell Line
Glucose - metabolism
Glucose uptake
GLUT-4
Insulin - pharmacology
Insulin resistance
IRS-1
Muscle Cells - drug effects
Muscle Cells - metabolism
Oxidation-Reduction - drug effects
Oxidative Stress
Rats
Reactive Oxygen Species - metabolism
Vitamin E - pharmacology
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Summary:The effect of reactive oxygen species (ROS) on insulin action is unclear. This study was carried out to explore the effect of prolonged low grade oxidative stress and vitamin E treatment on cultured rat L6 muscle cells. L6 myotubes were incubated with vitamin E for 18 h and treated with H 2O 2 generating system for 12 h. Insulin stimulated glucose uptake, total antioxidant capacity and reduced glutathione concentration were measured. There was a marked decrease in insulin stimulated glucose transport in L6 cells exposed to H 2O 2 generating system. Pretreatment with vitamin E attenuates the effect of H 2O 2 on insulin action. Treatment with H 2O 2 caused a significant reduction in the levels of reduced glutathione and total antioxidant capacity, these alterations were reversed by vitamin E pretreatment. Vitamin E per se had no effect on insulin stimulated glucose transport in cells not exposed to oxidative stress. In conclusion, our observations indicate that vitamin E improves the free radical defense system potential and prevents oxidative stress induced insulin resistance in rat L6 muscle cells.
ISSN:0009-8981
1873-3492
DOI:10.1016/j.cca.2005.12.004