Receptor for advanced glycation end products (RAGE) and its ligand, amphoterin are overexpressed and associated with prostate cancer development

BACKGROUND Advanced glycation end products (AGE) are produced with normal aging. Recently, some reports indicated that the interaction between AGE and the cognate receptor (RAGE) has a role in cancer dependent. METHODS We investigated RAGE and amphoterin mRNA expression in prostate cancer cell lines...

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Published in:The Prostate 2005-06, Vol.64 (1), p.92-100
Main Authors: Ishiguro, Hitoshi, Nakaigawa, Noboru, Miyoshi, Yasuhide, Fujinami, Kiyoshi, Kubota, Yoshinobu, Uemura, Hiroji
Format: Article
Language:English
Subjects:
amphoterin
Biological and medical sciences
Blotting, Western
Cell Line, Tumor
Cell Survival
Formazans
Gene Expression Regulation, Neoplastic
Glycation End Products, Advanced - metabolism
Gynecology. Andrology. Obstetrics
HMGB1 Protein - genetics
Humans
invasion
Ligands
Male
Male genital diseases
Medical sciences
Neoplasm Invasiveness
Nephrology. Urinary tract diseases
prostate cancer
Prostatic Hyperplasia - genetics
Prostatic Hyperplasia - pathology
Prostatic Hyperplasia - physiopathology
Prostatic Neoplasms - genetics
Prostatic Neoplasms - pathology
Prostatic Neoplasms - physiopathology
Receptor for Advanced Glycation End Products
Receptors, Immunologic - genetics
Receptors, Immunologic - metabolism
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - analysis
Tetrazolium Salts
Tumors
Tumors of the urinary system
Urinary tract. Prostate gland
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Summary:BACKGROUND Advanced glycation end products (AGE) are produced with normal aging. Recently, some reports indicated that the interaction between AGE and the cognate receptor (RAGE) has a role in cancer dependent. METHODS We investigated RAGE and amphoterin mRNA expression in prostate cancer cell lines (DU145, PC‐3, and LNCaP cells), hormone‐refractory prostate cancer tissues, and paired untreated primary prostate cancer and normal prostate (including benign prostatic hypertrophy (BPH)) tissues using real‐time quantitative PCR. Moreover, to confirm the AGE–RAGE interaction in prostate cancer, DU145 cells stimulated with AGE–bovine serum albumin (AGE–BSA) were examined by in vitro matrigel assay, cell viability assay, MTT assay, reverse transcription‐polymerase chain reaction (RT‐PCR), and Western blot. RESULTS DU145 cells, a hormone‐independent prostate cancer cell line, showed the highest RAGE mRNA expression. Amphoterin mRNA was expressed in all three cell lines. In prostate tissues, untreated prostate cancer tissue and hormone‐refractory prostate cancer tissue showed higher RAGE and amphoterin mRNA expression than normal prostate tissue. The AGE–RAGE interaction induced the invasion and growth in DU145 cells stimulated with AGE–BSA. CONCLUSIONS The AGE–RAGE interaction is important in prostate cancer development, and inhibition of this interaction has potential as a new molecular target for cancer therapy or prevention. © 2005 Wiley‐Liss, Inc.
ISSN:0270-4137
1097-0045
DOI:10.1002/pros.20219