Regionalization of the anterior hypothalamus in the chick embryo

Loss‐of‐function experiments in mice have shown that the transcription factors Sim1, Otp, Sim2, and Brn2 form a cascade essential for the differentiation of neuroendocrine cells of the anterior hypothalamus that produce vasopressin, oxytocin, somatostatin (SS), thyrotropin‐releasing hormone (TRH), a...

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Bibliographic Details
Published in:Developmental dynamics 2005-06, Vol.233 (2), p.652-658
Main Authors: Caqueret, Aurore, Coumailleau, Pascal, Michaud, Jacques L.
Format: Article
Language:English
Subjects:
Animals
Biomarkers
Cell Differentiation
Cell Nucleus - genetics
Chick Embryo
Gene Expression Regulation, Developmental
hypothalamus
Hypothalamus, Anterior - cytology
Hypothalamus, Anterior - embryology
Hypothalamus, Anterior - metabolism
In Situ Hybridization
Nerve Tissue Proteins - genetics
neuroendocrine cells
Repressor Proteins - genetics
RNA, Messenger - analysis
RNA, Messenger - genetics
transcription factors
Transcription Factors - genetics
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Summary:Loss‐of‐function experiments in mice have shown that the transcription factors Sim1, Otp, Sim2, and Brn2 form a cascade essential for the differentiation of neuroendocrine cells of the anterior hypothalamus that produce vasopressin, oxytocin, somatostatin (SS), thyrotropin‐releasing hormone (TRH), and corticotropin‐releasing hormone (CRH). Very little is known about how the differentiation of these cell types is regulated in chick. Here, we report the cloning of the chick homolog of Otp. Moreover, we have systematically compared the expression of Sim1, Sim2, Brn2, and Otp with that of the markers of terminal differentiation TRH, SS, CRH, vasotocin, and mesotocin during development of chick embryos. We have found that the cell types studied generally develop in domains expressing these transcriptional regulators but that the pattern of neuronal differentiation and the spatial distribution of some regulators were not the same as in mice. Our results provide a framework useful for the functional analysis of hypothalamus development in chick. Developmental Dynamics 233:652–658, 2005. © 2005 Wiley‐Liss, Inc.
ISSN:1058-8388
1097-0177
DOI:10.1002/dvdy.20372